外源性H_2S对氯胺酮全麻大鼠主要生理指标的影响

Effects of Exogenous H_2S on the Main Physiological Parameters in Rats Anesthetized with Ketamine

外源性H2S在诸多方面对中枢神经系统具有保护性作用,本研究通过预试验确定了外源性H2S的给药时间和剂量,将48只SD大鼠随机均分为4组,分别为对照组(CP组)、低剂量组(LP组,7μmol/kg NaHS)、中剂量组(MP组,14μmol/kg NaHS)、高剂量组(GP组,28μmol/kg NaHS)。在盐酸氯胺酮麻醉前20min,3个剂量组分别腹腔注射7、14、28μmol/kg NaHS,观察外源性H2S对大鼠体温、心率、呼吸频率、血氧饱和度及血压等生理指标的干预效果。试验结果表明,与对照组相比,3个剂量组体温和血氧饱和度浓度均显著升高(P<0.05),心率和血压均显著降低(P<0.05),但呼吸频率呈先减慢后加快趋势,且这些变化呈显著的剂量依赖性。

Exogenous H2 S, in many aspects, had a protective effect on the central nervous system. In preliminary experi ment, the administration time and dosage of exogenous H2 S were determined, and then 48 SD rats were randomly divided into four groups, such as control group （CP group）, low-dose group （LP group, 7 ~mol/kg NariS）, middle-dose group （MP group, 14 μmol/kg NariS）, high dose group （GP group, 28 μmol/kg NariS）. Animals in three dose groups were randomly administrated 7, 14, 28 μmol/kg Naris by intraperitoneal injection, followed the administration of ketamine 20 minutes after. The body temperature, heart rate, respiratory rate, blood oxygen saturation and blood pressure were recorded. The results showed that compared to the CP group, body temperatures and blood oxygen saturations in the dose groups significantly in creased （P〈0.05）, heart rates and blood pressures in the dose groups significantly decreased （P〈0.05）, respiratory rates in the dose groups slowed down, followed a tendency to increase, and these changes showed a significant dose dependent.