汶上芦花鸡催乳素基因外显子2的遗传变异及其与繁殖性状的相关性分析

Exon 2 SNP Detection of PRL Gene and its Associations with Egg Production Traits in Wenshang Barred Chicken

本研究以汶上芦花鸡为研究对象,利用PCR-SSCP技术对催乳素(prolactin,PRL)基因外显子2进行SNP检测和测序分析,并对该基因与汶上芦花鸡繁殖性状的相关性进行了分析。结果表明,PRL基因外显子2存在1个多态性位点,该位点由于在3838bp发生C→T的碱基突变,产生了AA、AB和BB 3种基因型,并导致氨基酸发生改变,由亮氨酸变为苯丙氨酸。BB基因型和等位基因B的频率最高;3种基因型(AA、AB和BB)在开产日龄、开产蛋重、开产体重、300日龄体重、300日龄蛋重的繁殖性状指标上均未达到差异显著性水平(P>0.05)。表明PRL基因外显子2多态性与汶上芦花鸡繁殖性状无明显相关性。

In this study, Wenshang Barred chickens was selected as the research object, exon 2 of prolactin （PRL） gene were detected by PCR-SSCP and the DNA fragments were sequenced. The correlation analysis between the polymorphism of the exon 2 and egg production traits were carried out respectively. The results indicated that there were two polymorphic sites in exon 2. C→T mutation at the position of the 3838 bp was found, which resulted in 3 genotypes （AA, AB and BB）. Further- more, an amino acid changed from leucine to phenylalanine. The frequencies of genotype BB and allele B were the highest. Three genotypes of AA, AB and BB in age at first egg, living weight at first egg, egg weight of first egg, living weight at age of 300 days, egg weight at age of 300 days index of reproductive traits difference did not reach the significant level （P〉0.05）. The results showed that PRL gene exon 2 polymorphism and reproductive traits of Wenshang Barred chickens presented no sig- nificant correlation.