犊牛腹泻大肠杆菌的分离鉴定和耐药性检测

Isolation,Identification and Resistance Testing of E.coli Isolated from Diarrheal Calves

为了分析南京地区奶牛场犊牛腹泻的原因,对犊牛腹泻病料进行细菌分离培养,获得了27株细菌。本试验对这些细菌进行了培养特性、染色特性、生化特性、血清型鉴定、药敏试验和TEM、SHV、CTX-M-1、CTX-M-9型超广谱β-内酰胺酶(ESBLs)基因的检测。结果显示27株细菌均为大肠杆菌,只有1株细菌对头孢噻吩、头孢唑啉和头孢呋辛耐药,且此菌株检测出CTX-M-1型超广谱β-内酰胺酶基因。试验结果表明犊牛腹泻是由大肠杆菌引起的,犊牛腹泻大肠杆菌能产生CTX-M-1型超广谱β-内酰胺酶,导致对头孢类抗生素耐药性的发生。

In order to analyze the causes of calf diarrhea in Nanjing, 27 strains bacteria were isolated from the material of di- arrhea calves. The cultured characteristics, staining characteristics, biochemical characteristics, serotype characteristics, drug susceptibility and TEM, SHV, CTX-M-1, CTX-M-9 type extended-spectrum 13-1actamase （ESBLs） gene of 27 strains bacteria were detected. The results showed that 27 strains bacteria were all E. coli, and only one bacteria was resistant for cephalothin, cefazolin and cefuroxime~ and this strain was detected in CTX-M-1 type extended spectrum 13 lactamase gene. The results showed that calf diarrhea were caused by E. coli, the calf diarrhea E. coli could produce CTX-M 1-type extended-spectrum 13- lactamase to resolve cephalosporin antibiotic that led to drug resistance.