高效液相色谱法同时测定补肾化瘀浸膏中淫羊藿苷及柚皮苷含量

Simultaneous Determination of Icariin and Naringin in Bushen Huayu Extract by HPLC

目的：建立高效液相色谱法同时测定补肾化瘀浸膏中淫羊藿苷及柚皮苷含量。方法 C18柱（4.6 mm×250 mm,5μm）,以乙腈-磷酸溶液（0.2%磷酸,pH=2.9）为流动相,梯度洗脱,流速0.8 mL·min^-1,检测波长269 nm,柱温30℃。结果淫羊藿苷在0.3～3.0μg范围内线性关系良好（r=0.9999）,加样回收率105.6%,RSD为0.95%；柚皮苷在0.12～1.20μg范围内线性关系良好（r=0.9999）,加样回收率94.0%,RSD为0.52%。结论该方法操作简便,准确稳定,重复性好,可作为补肾化瘀浸膏的质量控制标准。

Objective To establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC. Methods Reverse-phase high performance liquid chromatography （ RP-HPLC ） separation was performed one C18 column （4.6 mm×250 mm,5 μm）.The mobile phase was acetonitrile containing 0.2%H4PO3（pH=2.9）. Gradient elution with a flow rate of 0. 8 mL＆#183;min-1 was applied to achieve the separation. The detection wavelength was set at 269 nm,and the column temperature was 30℃. Results Icariin had a good linear range from 0. 3-3. 0μg （r=0. 999 9）,the recovery rate was 105. 6%,and RSD was 0. 95%. Naringin was linear within the range of 0. 12-1. 20 μg （r=0. 999 9）. The recovery rate was 94. 0%and RSD was 0. 52%. Conclusion The method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.