齐墩果酸对HepG2细胞胰岛素抵抗的影响

Effect of Oleanolic Acid on Insulin Resistance of HepG2 Cells

目的:采用高浓度胰岛素体外诱导培养HepG2细胞,建立胰岛素抵抗的细胞模型,观察齐墩果酸对HepG2细胞胰岛素抵抗的影响。方法:用CCK-8法筛选齐墩果酸作用于HepG2细胞的实验浓度,然后用高浓度胰岛素诱导HepG2细胞胰岛素抵抗,给予不同浓度的齐墩果酸(0.1、1、10、100μmol·L-1)、吡格列酮(10μmol·L-1)干预,以葡萄糖氧化酶法检测葡萄糖消耗量。结果:1不同浓度齐墩果酸对HepG2细胞增殖无明显影响。2与对照组比较,高浓度胰岛素作用24 h后,模型组葡萄糖消耗量明显降低(P<0.01);与模型组比较,齐墩果酸组HepG2细胞葡萄糖消耗量随浓度增加而增加,成一定的量效关系,浓度为10μmol·L-1和100μmol·L-1时葡萄糖消耗量显著增加(P<0.01或P<0.05)。结论:齐墩果酸能改善高浓度胰岛素诱导的HepG2细胞胰岛素抵抗。

Objective ： To establish the insulin resistant model of HepG2 cells in vitro induced by high insulin level and then to investigate the effects of oleanolie acid （OA） on insulin resistance in HepG2 cells. Methods：First of all,the con- centration of OA - treating HepG2 cells in the experiment was screened by CCK - 8 assay, and then insulin resistant mod- el was established by inducing HepG2 cells with high insulin level. Intervention of OA at different concentrations （0.1,1, 10,100μmol L-1） and pioglitazone （10μmol L-1） was performed at the same time. Glucose consumption was deter- mined by glucose oxidase method. Results：（1）Different concentrations of OA had no significant effect on proliferation of HepG2 cells. （2）Compared with control group, glucose consumption in model group was reduced significantly after the intervention of high insulin for 24 hours（ P 〈 0.01 ）. Compared with model group, glucose consumption in OA group was increased with increasing concentration, into a quantity -efficiency relation, in the concentrations of 10 μmol L-1 and 100 μmol L-1, glucose consumption increased significantly （P 〈0.01 or P 〈0.05）. Conclusion：OA can improve high in- sulin level- induced insulin resistance in HepG2 cells.