MSC治疗小鼠OB模型排斥反应的作用研究

Effect study of rejection of MSC in treatment of mice OB model

目的研究间充质干细胞(MSC)免疫抑制功能在治疗小鼠闭塞性细支气管炎(OB)中的作用。方法分离C57BL/6小鼠骨髓MSC,建立小鼠气管移植后OB反应模型,移植当天给予MSC,30 d后处死小鼠,移植气管HE染色,观察移植气管急性排斥反应的发生情况。并用酶联免疫吸附试验(ELISA)检测移植排斥相关炎症因子的含量。结果 MSC治疗移植组与异系移植组相比,排斥反应得到缓解,与无排斥反应的同系移植对照组相似,移植气管管腔阻塞程度缓解。移植气管ELISA检测发现,MSC治疗移植组小鼠体内炎症因子IFN-g下降,而抑炎因子白细胞介素-10(IL-10)上升。结论 MSC能缓解小鼠气管移植后OB反应,并调节体内免疫状态,抑制炎症。

Objective To analyze the effect of immunosuppression of mesenchymal stem cell （MSC） in the treatment of mice obliterans bronchiolitis （OB）. Methods Marrow MSC of C57BL/6 mice was separated to set OB reaction model after trachea transplantation of mice. MSC was given on the day of transplantation. The mice were put to death after 30 days. HE staining was used on the transplanted trachea, and the situation of acute rejection of transplanted trachea was observed. Enzyme-linked immuno sorbent assay （ELISA） was applied to detect the content of inflammatory factors related to transplant rejection. Results Rejection reaction in MSC transplant group was eased, compared with different system transplant group, and degree of lumen obstruction in transplanted trachea was also eased, compared with allografts transplant control group without rejection. ELISA test for transplanted trachea showed that inflammatory factor IFN-g decreased in MSC transplant group, while anti-inflammatory factor interleukin-10 （IL-10） increased. Conclusion MSC can ease the OB reaction after trachea transplantation, adjust immune state and inhibit inflammation.