17β-雌二醇对小鼠肺成纤维细胞细胞周期的影响

Effects of 17β-estradiol on mouse lung fibroblast cell cycle

目的:观察17β-雌二醇(17β-E2)对小鼠肺成纤维细胞细胞周期的影响。方法:根据L9(33)正交实验设计,用不同质量浓度SiO2(20、50、100 mg/L)诱导小鼠肺成纤维细胞,再用不同浓度17β-E2(10-6、10-7、10-8mol/L)干预不同时间(24、48、72 h),MTT法检测小鼠肺成纤维细胞的增殖状况,以增殖活性较低为筛选标准,选取最佳实验条件。将小鼠肺成纤维细胞分成3组:空白对照组、SiO2诱导组和17β-E2干预组,在相应实验条件下培养细胞,运用流式细胞术检测细胞周期。结果:最佳实验条件筛选结果为:用20 mg/L SiO2诱导,用10-6mol/L 17β-E2干预24 h。3组G1期细胞比例、S+G2期细胞比例差异均有统计学意义(F=50.300、29.896,P<0.001)。SiO2诱导组G1期细胞比例较空白对照组降低,S+G2期细胞比例升高;而17β-E2干预组G1期细胞比例较SiO2诱导组升高,S+G2期细胞比例降低(P<0.05)。结论:17β-E2可能是通过阻滞小鼠肺成纤维细胞由G1期进入S期和G2期,从而抑制其增殖。

Aim： To observe the effect of 17β-estradiol（ 17β-E2） on mouse lung fibroblast cell cycle. Methods： According to the L9（ 33） orthogonal experimental design,mouse lung fibroblasts were induced by SiO2（ 20,50,100 mg/L）,intervented by 17β-E2（ 10- 6,10- 7,10- 8mol /L） for 24,48,or 72 h. The cell proliferation was determined by MTT method. Based on low proliferation activity,the best experimental condition was selected. Mouse lung fibroblasts was divided into three groups： blank control group,SiO2 induction group,and 17β-E2 intervention group. The cells were cultured under the selected experimental condition,and the cell cycle was detected using flow cytometry. Results： The selected experimental condition was as follows： 20 mg /L SiO2 induction,and followed by 10- 6mol /L 17β-E2 intervention for 24 h.There were statistical differences in G1 phase cell proportion,and S ＋ G2 phase cell proportion among the three groups（ F =50.300,29.896,P〈0. 001）. Compared with blank control group,G1 phase cell proportion in SiO2 induction group was lower（ P〈0. 05）,while S ＋ G2 phase cell proportion was higher（ P〈0. 05）. Compared with SiO2 induction group,G1 phase cell proportion in 17β-E2 intervention group was higher（ P〈0. 05）,while S ＋ G2 phase cell proportion was lower（ P〈0. 05）. Conclusion： 17β-E2 might inhibit mouse lung fibroblast proliferation by blocking the cells in G1 phase.