杨梅乙醇提取物对小鼠酒精性肝损伤的保护作用

The Protective Effect of Ethanol Extract of Chinese Bayberry Fruit on Alcohol-induced Liver Injury in Mice

目的:探明杨梅乙醇提取物对小鼠酒精性肝损伤的保护作用。方法:采用动物试验研究不同剂量的杨梅乙醇提取物对小鼠肝组织、线粒体和微粒体中主要非酶和酶类抗氧化剂、氧化剂以及丙二醛的影响。结果:与酒精模型组比较,高剂量(200 mg/kg)组小鼠肝组织中的一氧化氮(NO)、一氧化氮合成酶(NOS),肝线粒体和微粒体中的丙二醛浓度显著降低(P<0.05),而肝组织中的还原型谷胱甘肽浓度(GSH)、乙醇脱氢酶(ADH)、总抗氧化能力(T-AOC),线粒体中的过氧化氢酶(CAT),微粒体中的GSH浓度、谷胱甘肽-S-转移酶(GST)、过氧化物酶(POD)显著升高(P<0.05),且呈明显的剂量-效应关系。结论:杨梅乙醇提取物可增强酒精性肝损伤小鼠机体的抗氧化性,抑制氧化应激和脂质过氧化,对酒精性肝损伤有保护作用。

Objective： The objective of the study is to investigate the protective effect of flavonoids extract against alcoholic liver injury in mice. Method： The effect of different dosage （50-200 mg/kg） of flavonoids extract on the main enzymatic and non-enzymatic antioxidants, oxidants as well as the level of malondialdehyde （MDA） in mice were studied. Result： The results showed that, compared with alcohol group, the levels of nitric oxide （NO） and nitric oxide synthase （NOS） in the liver tissue reduced remarkably （P〈0.05） in the group supplement with 200 mg/kg, together with MDA content in liver mitochondrion and microsome, whereas the reduced glutathione （GSH）, alcohol dehydrogenase （ADH） and total antioxidant capacity（T-AOC） in liver tissue elevated significantly （P〈0.05）, so were activities of catalase （CAT） in liver mitochondrion and GSH, glutathione-S-transferase （GST） as well as peroxidase （POD） in liver microsome. And it was also closely dose-dependent. Conclusion： The above results indicated that flavonoids extract could enhance the an- tioxidant abilities in alcohol-induced liver injury in mice and prevented oxidative stress and lipid peroxidation mediated by alcohol. These findings suggested that flavonoids extract exerted hepatoprotective activity from alcoholic liver injury.