八珍汤中芍药苷的质量浓度测定

Determination of paecniflorin of Bazhen decoction

建立八珍汤中芍药苷的高效液相色谱定量方法．HPLC分析采用Symmetry C18色谱柱（4．6 mm ×250 mm，5μm）；紫外检测器，检测波长为230 nm；流动相为乙腈-0．05％磷酸水溶液（15∶85）；流速为1．0 mL/min；柱温为30℃；进样量为20μL．芍药苷在0．014～0．224 mg/mL范围内呈良好线性关系，线性回归方程为Y ＝40798281 X ＋2855，r ＝0．9998（n＝5）．该方法具有分析速度快，重现性好，精密度高，定量准确等特点．适用于八珍汤复方中芍药苷的定量分析．

To establish an HPLC method for determination of paecniflorin of Bazhen decoction the assay was performed on a Symmetry C 18 column （250 mm ×4.6 mm, 5μm）.UV detec-tor wavelength was at 230 nm.The mobile phase was acetonitrile -0.05%phosphoric acid solution （15∶85）.The flow rate was 1.0 mL/min, and the column temperature was main-tained at 30 ℃.The sampling volume was 20μL.The calibration curve had good linear re-lationship in the range of 0.014~0.224 mg/mL, linear equation： Y =40 798 281 X ＋2 855（r=0.999 8）.This method was simple, sensitive, accurate and reproducible .It was suitable for quality control of Bazhen decoction .