虎眼万年青体内抗氧化性的研究

Study on antioxidant activity of Ornithogalum caudatum Ait in vivo

研究虎眼万年青乙醇提取物体内抗氧化性的作用.利用加热回流提取方法提取虎眼万年青总皂苷,分别以高中低(30、15、7.5 mg/kg)三种不同剂量的虎眼万年青乙醇提取物,对小鼠灌胃给药14d,并以Vc和水分别作阳性对照和阴性对照.通过测定小鼠血清和肝组织中的超氧化歧化酶(SOD)和丙二醛(MDA)值来研究虎眼万年青乙醇提取物体内抗氧化活性.结果表明,与阴性对照(蒸馏水)相比,虎眼万年青乙醇提取物能明显提高小鼠血清和肝组织中SOD的活力,降低MDA的含量(P<0.01);与阳性对照Vc相比,高剂量组血清中SOD的活力显著高于对照组(P<0.01),且肝组织中MDA的含量显著低于对照组(P<0.05).虎眼万年青乙醇提取物具有较强的体内抗氧化活性.

To study the antioxidant effect of ethanol extract of Ornithogalum caudatum Ait in vivo, total saponins of Ornithogalum caudatum Ait was extracted by heat reflux extraction . ethanol extracts of three different doses （30,15,7.5mg/kg） feed mice for 14 days.Vc solu-tion and water were used as positive control and negative control .Detecting numerical chan-ges of the mice serum, liver tissue of malondialdehyde （ MDA） and super oxide dismutase （ SOD） , experimental data was used to analyze and study the tiger yield of ethanol extracts of antioxidant activity in the body .The results showed that compared with negative control （ dis-tilled water ） , ethanol extract of Ornithogalum caudatum Ait could increase serum and liver tissue SOD, decrease MDA content （P 〈0.01）.Compared with the positive control Vc group, a higher concentration of SOD in serum （P 〈0.05） and MDA in liver tissue （P 〈0.05） was detected in high dose group （30 mg/kg） .The results showed that the ethanol ex-tract of Ornithogalum caudatum Ait showed good antioxidant activity .