HPLC-RID法同时测定复方痱子粉中4种成分的含量

Determination of Four Components in Compound Miliaria Powder by HPLC-RID

目的 建立高效液相色谱-示差折光检测法测定复方痱子粉中樟脑、麝香草酚、薄荷脑和克霉唑4种成分的含量。方法 采用示差折光检测器,C18色谱柱（150×4.6mm,5μm）,以甲醇-水（78：22）为流动相,柱温30℃,流速为0.8mL/min,进样量为20μL。结果 线性范围分别为樟脑0.210~0.631mg/mL,r=0.999 9;麝香草酚0.312~0.935mg/mL,r=0.999 8;薄荷脑0.207~0.621mg/mL,r=0.999 9;克霉唑1.389~4.166mg/mL,r=0.999 9。平均回收率为樟脑100.4%（RSD=0.5%）,麝香草酚99.5%（RSD=0.7%）,薄荷脑100.4%（RSD=0.6%）,克霉唑100.3%（RSD=0.5%）。结论 本法简便,快速,准确,可作为本品质量评价和生产工艺监控的有效方法。

Objective To establish an HPLC-R1D method to deternline four components in compound miliariapowder. Methods A C18 column （150×4.6mm, 5μm） was used, the column temperature was set at 30℃, the mobilephase was consisted by methanolwater（78：22）, and flow rate was 0.8 mL/min, the detection was conductedby RID. Results The calibration curves were linear in the range of 0.210-0.631mg/mL（r=0.999 9）lor camphor, 0.312-0.935mg/mL（r=0.999 8） for thymol, 0.207-0.621mg/mL（r=0.999 9） for menthol and1.389-4.166mg/mL（r=0.999 9） for clotrimazole. The average recoveries for the assay were 100.4% （RSD=0.5%）,99.5% （RSD=0.7%）, 100.4% （RSD=0.6%） and 100.3% （RSD=0.5%）, respectively. Conclusion This proposedmethod appeared to be simple, rapid and accurate, and can be used to control the product quality and preparationprocess.