高效液相色谱法同时测定珠子参中4种化学成分的含量

HPLC simultaneous determination of 4 chemical components in Panacis Majoris Rhizoma

目的建立不同产地加工珠子参中人参皂苷Ro、竹节参皂苷Ⅳa、姜状三七苷R1和去葡萄糖竹节参皂苷Ⅳa 4种化学成分的含量测定方法。方法采用Kromasil C18（4.6 mm×250 mm,5μm）色谱柱;以0.2%的磷酸溶液和乙腈进行梯度洗脱;柱温25℃;流速1 mL·min-1;检测波长203 nm。结果在HPLC法中,人参皂苷Ro、竹节参皂苷Ⅳa、姜状三七苷R1和去葡萄糖竹节参皂苷Ⅳa的线性范围分别是：0.81-16.20μg（r=0.999 9）、0.46-9.20μg（r=1.000 0）、0.38-7.60μg（r=0.999 9）、0.16-3.20μg（r=0.999 9）;平均回收率分别为100.3%、99.1%、100.1%、95.8%,RSD分别为2.6%、2.3%、2.5%、0.7%。结论该方法专属性强,重复性好,可用于不同加工方法珠子参的质量控制。

Objective To establish an HPLC method to simultaneously determinate 4 chemical components of ginsenoside Ro, chikusetsusaponin Ⅳ a, zingibroside R1 and deglucose chikusetsusaponin Ⅳ a in Panacis Majoris Rhizoma. Methods The chromatograhic separation was Kromasil C18 column（4.6 mm×250 mm, 5 μm）; the column temperature was maintained at 25 ℃; A linear elution of 0.2% phosphoric acid aqueous solution-acetonitrile was adopted at 1 mL·min- 1. The. UV detection wavelength was 203 nm. Results Ginsenoside Ro, chikusetsusaponin Ⅳ a, zingibroside R1 and deglucose chikusetsusaponin Ⅳ a had good linearity at 0.81- 16.20 μg（r = 0.999 9）, 0.46-9.20 μg（r = 1.000 0）, 0.38- 7.60 μg（r = 0.999 9） and 0.16- 3.20 μg（r = 0.999 9）, respectively. The average recoveries were 100.3%（2.6%）, 99.1%（2.3%）, 100.1%（2.5%） and 95.8%（0.7%）, respectively. Conclusion This method is simple, accurate and reproduceable, which can be used for the quality control of Panacis Majoris Rhizoma.