miRNA-126基因敲减小鼠肠系膜淋巴结T淋巴细胞比例的变化

Change on proportion of T lymphocytes in mesenteric lymph nodes in miRNA-126 knockdown mice

目的:研究miRNA-126基因敲减小鼠肠系膜淋巴结T及B淋巴细胞数量的变化。方法:检测miRNA-126基因敲减(KD)小鼠肠系膜淋巴结的体积、重量和淋巴结细胞总数;HE染色检测小鼠肠系膜淋巴结的病理形态学变化;流式细胞术(FACS)检测淋巴结中T及B淋巴细胞数量的变化。结果:与野生型(WT)小鼠相比,miR-126KD小鼠肠系膜淋巴结体积、重量和淋巴结细胞总数均显著增加(P<0.05),且病理形态学发生异常;肠系膜淋巴结中B淋巴细胞比例和数量无变化(P>0.05),CD4+T细胞数量无变化,而CD4+T细胞百分数明显减少(P<0.05),CD8+T细胞百分数和绝对数量均显著增加(P<0.05)。结论:miRNA-126敲减后可显著影响肠系膜淋巴结中T淋巴细胞的数量,提示miRNA-126可能影响肠系膜淋巴结的免疫功能。

Objective：To detect the change on proportion of T and B lymphocytes in mesenteric lymph nodes in miRNA-126 （miR-126） knockdown mice. Methods： The volume,weight and total cells number of mesenteric lymph node in miR-126 knockdown （KD） mice were measured;the pathologic morphology change of mesenteric lymph nodes was observed by HE staining;and the change on proportion of T and B lymphocytes in mesenteric lymph nodes in miR-126KD mice were analyzed by Flow cytometry. Results： Compared with those of WT mice, the volume and weight, as well as total cells number of mesenteric lymph node were significantly increased （P〈0. 05 ）. Moreover, the pathologic morphology was significantly abnormal. The proportion and numbers of B lymphocyte, as well as absolute numbers of CD4~ T cells did not change significantly （P〉0. 05 ）. However, the proportion of CD4＋ T cells was remarkably decreased （P〈0. 05 ）; moreover,the proportion and absolute numbers of CD8＋ T cells were significantly increased（P〈 0. 05 ）. Conclusion ： There is significant influence in the numbers of T lymphocytes in mesenteric lymph nodes in miR-126KD mice, suggesting that miR-126 might play an important role in immunological function.