肿瘤细胞裂解物联合化脓性链球菌裂解物的抗肿瘤作用

Anti-tumor immunity of tumor cell lysate combined with group A streptococcus lysate induce in mice

目的:研究化脓性链球菌裂解物与肿瘤细胞裂解物联用,抑制荷瘤小鼠肿瘤的生长。方法:自行制备了化脓性链球菌裂解物制剂,通过体外及动物实验测定其抗肿瘤效应。以BALB/c小鼠EMT6乳腺癌为模型,观察了链球菌制剂联合肿瘤细胞裂解物对荷瘤小鼠肿瘤生长的影响。结果:自行制备的化脓性链球菌在体外可以刺激小鼠脾细胞的增殖,且能抑制多种肿瘤细胞(EMT6、B16、A549)的增殖,二者联用对每只荷瘤小鼠的肿瘤体积都有较强的抑制作用(P<0.05)。结论:化脓性链球菌裂解物与肿瘤细胞裂解物联用可以抑制荷瘤小鼠肿瘤的生长。

Objective： To develop new vaccine for treatment of breast cancer. Methods ： We have prepared the Group A Strep- tococcus pyogenes （GAS） lyaste and tumor cell lysate and evaluated their anti-tumor activity in vitro and in a breast cancer mouse model. Results ： Interestingly, GAS lysate, prepared in this study, showed a vigorous activity on the stimulation the proliferation of mouse spleen cells and inhibition of the growth of variety of tumor cells in vitro, such as EMT6, B16 and A549. In addition, the mixture of GAS lysate and tumor cell lysate could significantly inhibit tumor growth in mice treated with EMT6 cell （ P〈0. 05 ）. Conclusion ： The data suggest that the mixture of GAS lysate plus tumor cell lysate might be developed into a candidate tumor vaccine for the treatment of breast cancer