类风湿关节炎滑膜组织RAGE的表达及意义

Expression and clinical significance of RAGE in rheumatoid arthritis

目的:探讨晚期糖基化终末产物受体(RAGE)在类风湿关节炎滑膜组织中的表达与临床意义。方法:收集2009年7月至2013年12月在我院接受手术治疗的类风湿关节炎患者(20例)术中切除的滑膜组织标本,以及骨关节炎患者(16例)的关节滑膜组织,通过免疫印迹法和反转录聚合酶链(RT-PCR)反应法检测RAGE的mRNA和蛋白在类风湿关节炎滑膜组织中的表达量,并与下游相关炎性因子TNF-α和IL-1β的表达做相关性分析。结果:与骨关节炎组相比,RAGE的mRNA(P=0.001)和蛋白(P=0.000 4)表达量明显增加,下游炎性因子IL-1β(P=0.003)和TNF-α(P=0.005)的表达量也明显增加,两种炎性因子的含量与RAGE的表达量存在显著正相关性(IL-1β:r=0.768,P=0.012;TNF-α:r=0.836,P=0.008)。结论:RAGE是类风湿性关节炎发病机制中的重要参与者,靶向RAGE的治疗可能对于改善类风湿性关节炎的预后具有重要的意义。

Objective： To determine the expression of receptor for advanced glycation end products in synovial tissue from rheumatoid arthritis （RA）. Methods： Synovial tissue samples were obtained from2Opatients with RA and 16 patients with osteoarthritis （OA） in our hospital between 07.2011 and 12.2013. Messenger RNA （mRNA） expression of RAGE was examined by reverse transeriptase-polymerase chain reaction （PCR）. Western blot was used to detect the protein expression of RAGE in synovial tissue. The relationship between the cytokines and RAGE expression in RA patients was also evaluated. Results： RAGE mRNA（ P= 0. 001 ） and protein （P = 0. 000 4 ） expression was markedly greater in RA than in OA lesions （P〈0. 05 ）. In RA joints, levels of IL-1β （P = 0. 003 ） and TNF-α （P = O. 005 ） were strikingly increased compared with OA patients. In addition, the synovial levels of RAGE are comparable with those of IL-1β （ r = 0. 768, P = 0. 012 ） and TNF-α （ r = 0. 836, P = 0. 008 ） in RA patients. Conclusion ： RAGE may play an important role in the pathology and development of RA, and could be an important therapeutic target in the treatment of RA.