甘氨双唑钠通过下调ATM表达实现对喉癌细胞的放射增敏效应

Sodium Glycididazole Enhancing Radiosensitivity of Laryngeal Cancer Cells through Down-Regulation of the ATM Expression

目的探讨甘氨双唑钠对放射线照射后喉癌细胞AT突变基因(AT mutated,ATM)表达的影响。方法将喉癌Hep-2细胞分为空白组(不做任何处理)、照射组(接受5 Gy X线照射)、观察组(甘氨双唑钠+照射组),经浓度为0.8 mmol/L甘氨双唑钠100μl作用4 h后,再接受5 Gy X线照射,分别采用逆转录聚合酶链反应(RT-PCR)和蛋白免疫印迹杂交技术检测ATM mRNA及蛋白表达量。结果空白组、照射组、观察组ATM mRNA相对表达量分别为0.727±0.024、0.955±0.046、0.272±0.038,3组间两两比较差异均有统计学意义(P<0.05);经甘氨双唑钠干预后喉癌Hep-2细胞ATM蛋白表达水平明显下降,3组间两两比较差异亦均有统计学意义(P<0.05)。结论甘氨双唑钠可能通过下调ATM的表达实现对喉癌细胞的放射增敏效应。

Objective To evaluate the expression of ataxia-telangiectasia mutated （ATM） gene in laryngeal cancer cells after the intervention of combined sodium glycididazole and radiation. Methods Laryngeal cancer Hep-2 cells were di-vided into the normal group, the irradiation group （5 Gy of X-radiation） and the observation group （irradiation plus sodium glycididazole group） . In observation group, laryngeal cancer cells received 5 Gy of X-radiation at 4 h after the intervention of 100 μl of sodium glycididazole （concentration of 0. 8 mmol/L）. The ATM mRNA and protein expression of laryngeal cancer cells were detected by RT-PCR and Western blot hybridization, respectively. Results The ATM mRNA expression levels in normal group, irradiation group and observation group were 0. 727±0. 024, 0. 955±0. 046 and 0. 272±0. 038, respectively. The ATM mRNA and protein expression of laryngeal cancer Hep-2 cells declined after the intervention of sodium glycididazole. The differences of ATM mRNA and protein expression levels among the three groups were significant （P〈0. 05）. Conclusion Sodium glycididazole could enhance radiosensitivity of laryngeal cancer cells through down-regulation of the ATM expres-sion.