生物种衣剂SN101的研制及其对大豆胞囊线虫病的防效

Development of a biological seed-coating preparation and it's efficiency in prevention of Heterodera glycines

本研究利用微生物多样性生态平衡原理,研发了生物种衣剂SN101,并进行了田间试验,旨在解决大豆胞囊线虫病问题。采用自主筛选的对线虫有拮抗作用的细菌巨大芽孢杆菌Sneb482、简单芽孢杆菌Sneb545和费氏中华根瘤菌Sneb183菌株进行多菌株复配,通过对大豆种子发芽能力的检测试验,验证种衣剂的复配比例及对寄主植物的安全性。田间试验结果表明生物种衣剂SN101对大豆出苗无影响,对大豆胞囊线虫病防效(46.51%)显著高于商品种衣剂对照(重茬丹,主要成分为BFA生物制剂20%)和空白对照,对大豆株高、产量亦有明显的促生和增产作用。

Based on ecological balance theory of biodiversity,biological seed coating SN101 was carried out in field.The objective of this research was to eradicate Heterodera,to test the compounding proportion of seed coating and threats to host plant.Sneb482,Sneb545 and Snebl83 were antagonistical to nematode,and were compounded into different proportion.The germination of soybean was examined.Field efficiency trials carried out in 2012 and 2013.The rate of emergence in seedling stage were calculated.We counted the cysts outside the soybean roots and in the soil around the roots,and the cyst nematodes inside the roots of different stages from 30 to 35 days after see-ding,surveyed the influence on plant growth,tested soybean yield in autumn.The results showed that the biologi-cal seed coating SN101 had no effect on emergence of soybean seedings and significant better than no coated and commodity seed coating in fields.Biocontrol seed coating SN101 could not only promote soybean growth and devel-opment,but also effectively suppress soybean cyst nematode numbers around soybean roots and increase soybean yield.It showed great financial benefit and a promising future.