新型生物可降解雷帕霉素药物洗脱输尿管支架的构建及性能评估

Construction and performance evaluation of a new biodegradable rapamycin-eluting ureteral stent

目的构建一种新型生物可降解雷帕霉素药物洗脱输尿管支架并评估其机械性能及药物释放特性。方法采用聚乳酸为原料,将其溶于氯仿后加入雷帕霉素粉末,通过溶液挥发法构建螺旋形生物可降解雷帕霉素药物洗脱输尿管支架。通过万能压力机检测新型生物可降解雷帕霉素输尿管支架力学性能,并分别在3周、6周、9周和12周时通过色谱分析仪评估其药物释放特性。结果成功构建的生物可降解雷帕霉素药物洗脱输尿管支架,长度为50 mm,内径为0.8 mm,外径1.4 mm,厚度为200μm,带宽为1 mm。支架不仅具有良好的力学性能,并可有效持续释放药物,支架平均雷帕霉素含量为2 755μg,3周后支架平均含药量为2 670μg,6周后支架平均含药量为2 533μg,9周后支架平均含药量为1 541μg,12周后支架平均含药量为744μg。结论构建的生物可降解雷帕霉素药物洗脱输尿管支架具有良好的机械性能,可有效释放药物,有望为输尿管损伤或狭窄的治疗提供一种新的简单有效的材料和方法。

Objective To construct a new biodegradable rapamycin-eluting ureteral stent and to evaluate its mechanical property and drug-eluting characteristics. Methods Polylactic was selected as the stent material and dis-solved by chloroform. The rapamycin powder was added into the solution. Then, a new helical biodegradable rapa-mycin-eluting ureteral stent was made by volatilization method. The mechanical property of the stent was measured by universal tension tester, while the drug-eluting characteristic was evaluated by chromatographic analysis ma-chine. Results The new biodegradable rapamycin-eluting ureteral stent had good mechanical property with length in 50 mm, inner diameter in 8 mm, outer diameter in 1.4 mm, thickness in 200μm and width in 1 mm. The stent can continuously release drug. The mean amount of rapamycin of the stent was 2 755 μg. After 3 weeks, the remained mean amount of rapamycin was 2 670μg. At 6 weeks, it was 2 533μg and 1 541 at 9 weeks. Eventually, it was de-creased to 744μg at 12 weeks. Conclusion The new biodegradable rapamycin-eluting ureteral stent made of polylac-tic acid not only has good mechanical property, but also can release drug efficiently, which may provide a new materi-al for the treatment of ureteral strictures.