摘要
目的探讨丙型肝炎病毒(HCV)在体内外对白细胞介素32(IL-32)表达的影响。方法荧光定量PCR和免疫印迹法检测Huh7.5.1细胞中IL-32 mRNA和蛋白的表达水平,酶联免疫吸附试验(ELISA)检测IL-32的血清含量。结果 HCV感染的Huh7.5.1细胞IL-32 mRNA和蛋白的表达水平较对照细胞高,HCV患者IL-32血清含量明显高于健康对照(P<0.05)。结论 HCV能够在体内外促进IL-32的合成和分泌。
Objective To explore the effect of hepatitis C virus (HCV) on the expression of interleukin 32 (IL-32). Methods The mRNA and protein expression of IL-32 in Huh7.5.1 cells were measured by real-time PCR and western blot. Serum levels of IL-32 were measured by enzyme-linked immunosorbent assay (ELISA). Results Ex-pression of IL-32 mRNA and protein was higher in Huh7.5.1 cells infected by HCV than in the control cells. Serum IL-32 levels was significantly higher in HCV patients as compared with healthy controls (P〈0.05). Conclusion HCV can upregulate the synthesis and secretion of IL-32 both in vivo and in vitro.
出处
《海南医学》
CAS
2014年第18期2708-2709,共2页
Hainan Medical Journal
基金
国家临床重点专科专项经费(编号:2010305)
国家自然科学基金(编号:81101485)
湖北省教育厅科学技术研究项目(编号:D20122404)