江苏省急性弛缓性麻痹病例病原学监测

Pathogen surveilliance and analysis on acute flaccid paralysis cases in Jiangsu Province

目的掌握江苏省脊灰和非脊灰肠道病毒血清型别以及脊灰病毒的基因型别,确认其是否野毒株,为维持无脊灰状态提供病毒学依据。方法对2005-2013年江苏省急性弛缓性麻痹病例(AFP)监测数据进行分析,由国家脊灰实验室对脊灰病毒株进行型内鉴定。结果 2005-2013年江苏省脊灰实验室共收到AFP病例粪便标本2 413份,AFP病例接触者粪便标本252份。AFP病例中分离到脊灰病毒86株,年均分离率3.56%,分离到非脊灰肠道病毒163例,年均分离率6.76%;AFP病例接触者中分离出脊灰病毒12例,年均分离率4.76%,分离出非脊灰肠道病毒22例,年均分离率8.73%。未发现脊灰野病毒和疫苗衍生脊灰病毒。结论江苏省脊灰实验室为WHO和国家脊灰实验室盲样标本能力验证和现场认证评估合格实验室,细胞系敏感性均达到WHO要求,本监测数据可为继续保持无脊灰状态提供病毒学依据。

Objective To understand about serotypes of polio and non-polio enteroviruses and poliovirus （PV） genotype, to confirm whether the ridge of virus wild strains or vaccine strain so as to provide basis on maintaining polio-free status. Methods Surveilliance data were analyzed for reported acute flaccid paralysis （AFP） cases from 2005 to 2013 in Jiangsu Province. And strains of all polio virus type were identified by national polio laboratory. Results From 2005 to 2013, a total of 2 413 polio AFP cases stool specimens, 252 cases of AFP cases contact stool specimens were received by polio laboratory of Jiangsu Prov- ince. AFP cases isolated poliovirus （PV） 86 cases, with an average annual rate of 3.56%separation; 163 cases of non-polio enteroviruses isolated （NPEV）, an annual separation rate was 6.76% ; AFP cases stool specimens contacts PV isolated 12 cases, the average separation rate was 4. 76%, isolated NPEV 22 cases, with an average annual rate of 8.73% separation. No wild poliovirus and vaccine-derived poliovirus （VDPV） were found. Conclusion Jiangsu Polio Laboratory qualified by the World Health Organization （WHO） and national polio laboratory on proficiency testing samples, blind samples and site certification assessment, the sensitivity of cell lines also meet WHO requirements. The surveilliance data can provide virology evidence for maintaining polio-free status.