不同妊娠期振动对SD大鼠子代空间学习记忆的影响

Effects of different prenatal vibration stress on spatial learning and memory in rats

目的探讨不同妊娠期振动对大鼠子代空间学习记忆的影响。方法分别在SD大鼠妊娠的1-7d、8-14d、15-21d施加强弱交替的振动刺激,在子鼠60d使用Barnes迷宫测试其空间学习记忆能力,比较各组间空间学习记忆的差异。结果学习过程：1）探索路程：妊娠早期振动组在第4天,妊娠中期在第1天,妊娠晚期在第1天高于对照组,妊娠晚期在第2、3天低于对照组（P〈0.05）;2）探索平均速度：妊娠早期在第2天,妊娠中期在第1、2天,妊娠晚期在第1、2、3、7、8天均低于对照组（P〈0.05）;3）错误探索时间：妊娠早期在第1、3、4、7、8天,妊娠中期在第3、4、5、8天高于对照组,妊娠晚期在第2、6天低于对照组（P〈0.05）;4）首次探索目标洞潜伏期：妊娠中期在第5天高于对照组（P〈0.05）。记忆过程：妊娠早期振动组的错误探索时间明显高于对照组（Mann-Whitney U=43.00,P=0.000）,妊娠晚期振动组的探索路程（Mann-Whitney U=150.00）、平均速度（Mann-Whitney U=150.00）和错误探索时间（Mann-Whitney U=164.50）明显低于对照组（P〈0.05或〈0.01）。结论妊娠早期振动损害子代的空间学习记忆能力,妊娠晚期振动可能促进子代的空间学习记忆能力,而妊娠中期仅损害子代的空间学习能力。

Objective To explore the effects of prenatal vibration stress in different gestational time points on the spatial learning and memory in rats. Methods The Sprague-Dawley （SD） pregnant rats were vibrated strong and weak alternately in 1 -7 d, 8- 14 d, 15 -21 d of gestation period, and the offspring was tested in Barnes maze to identify their spatial learning and memory,the difference of each group then compared. Results Learning process： 1）The journey of exploration：It was higher than control group during early pregnancy group was in 4 d, during middle pregnancy group in ld and during late pregnancy group in ld;It was lower than control group during late pregnancy group in 2 and 3 d （P〈0. 05） ;2） The average speed of exploration：h was lower than control group during early pregnancy group in 2 d,during middle preg- nancy group in 1 d and 2 d, during late pregnancy group in 1,2,3,7,8 d （P〈0. 05） ;3）The time of exploring errors holes：It was higher than control group during early pregnancy group in 1,2,4,7,8 d,during middle pregnancy group in 3,4,5,8d;lt was lower than control group during late pregnancy group in 2,6 d （P〈0.05）;4）The latency of exploring target hole：It was higher than control group during middle pregnancy group in 5 d （P〈0.05）. Memory process：The time of exploring errors holes was higher during early pregnancy group （Mann-Whitney U = 43. 00, P = 0. 000）;The journey of exploration （Mann-Whitney U=150.00） ,the average speed of exploring （Mann-Whitney U=150.00） and the time of exploring errors holes （Mann-Whitney U= 164.50） were lower during late pregnancy group（P〈0.05 or 〈0.01）. Conclusion The vibration stress in early pregnancy group may damage the spatial learning and memory ability, and it may promote the spatial learning and memory ability in the late pregnancy group; but the vibration stress in mid pregnancy group only damage the spatial learning ability.