舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应

Cloning and Analysis of LdOA1 in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides

G蛋白偶联受体(GPCRs)是生物体内重要膜受体,通过激活三聚体G蛋白参与各种细胞内信号转导,在药物研发中具有重要的作用。从舞毒蛾无参照全转录本文库中鉴定获得GPCR家族中眼白化I型全长基因(命名为LdOA1),该基因全长453 bp,编码150个氨基酸,分子质量为17.54 kDa,理论等电点(pI)为9.76。进化树分析表明LdOA1蛋白与帝王蝶的亲缘关系较近。实时荧光定量PCR分析表明舞毒蛾3龄幼虫在溴氰菊酯、甲萘威和氧化乐果胁迫下LdOA1的表达均表现为显著抑制。

The G protein coupled receptors （ GPCRs） ,known as transmembrane receptors,play an important role in drug research and development by activating G protein involved in inside signal transduction pathways. In present study, the full length cDNA of GPCR （namely LdOA1） was isolated from Lymantria dispar transcriptome. The open reading frame （ ORF） of LdOA1 was 453 bp encoding a protein of 150 amino acid residues with the molecular mass of 17. 54 kDa and theoretical pI of 9. 76. Phylogenetic analysis of GPCR proteins showed LdOA1 of L. dispar clustered into a group with Danaus plexippus. We further investigated the expression of LdOA1 in 3rd instar L. dispar larvae under deltamethrin, carbaryl and omethoate stresses using real-time PCR. The results showed that LdOA1 in L. dispar was obviously inhibited by deltamethrin,carbaryl and omethoate.