鱼腥草滴眼液眼局部应用的安全性研究

Research on the ocular safety of Houttuynia cordata Thunb eye drops application

目的研究鱼腥草滴眼液眼局部应用的安全性。方法 20只SD大鼠按照随机数字表法分为对照组和实验组,以生理盐水滴眼为对照,鱼腥草滴眼液滴眼为实验组,每日滴眼4次,共14天。观察局部点眼后大鼠的刺激症状,裂隙灯显微镜观察眼表组织的形态学变化,HE染色光镜检查大鼠角膜用药后眼表结膜、角膜组织的炎症发生情况。结果两组大鼠接受滴眼液点眼后,无明显行为学改变,无明显刺激症状。两组大鼠Draize刺激实验评分用χ2检验后发现差异无统计学意义(P>0.05)。裂隙灯观察未发现眼表和眼前节组织有明显异常。病理切片HE染色检查发现两组大鼠结膜和角膜上皮完整,基质排列有序规则,未发现明显炎性细胞浸润。结论鱼腥草滴眼液眼局部应用安全性良好。

Objective To evaluate the ocular safety of Houttuynia cordata Thunb eye drops appli- cation. Methods 20 SD rats were randomly divided into two groups ： the control group and the test group. In the control group, normal saline was used as the eye drops. In the test group, Houttuynia eordata Thunb eye drops were applied. The eye drops were given 4 times a day for 14 days. The behavior and irritation changes of the rats were observed. Slit lamp corneal microscope examination was used to analyze the chan- ges of the conjunctiva, cornea and iris. HE staining was used to analyze the changes of the ocular tissues. Results There were no changes in the behavior of the rats in both groups. There were no obvious irritation changes of the ocular tissues. There was no statistical difference in two groups on the scores from Draize system by X2 test. Slit lamp corneal microscope examination showed that there were no obvious changes in the ocular surface and anterior segment. HE staining demonstrated that in the ocular tissues in both groups were both abnormal after the application of eye drops. Conclusion The application of Houttuynia cordata Thunb eye drops on cornea was safe.