银杏叶复方制剂对小鼠急性毒性和细胞毒性研究

Study on acute toxicity in mice and cytotoxicities of Ginkgo biloba compound

文章旨在研究银杏叶复方制剂对小鼠急性毒性和细胞毒性的影响，以确保临床用药安全。结果表明，①急性毒性试验以半数致死量（LD50）和最大耐受量（MTD）为指标，用40 g·kg-1体重剂量的银杏叶复方制剂灌胃，7 d后小鼠未发生死亡，再在小鼠可接受的最大容积下，给小鼠灌服日累积剂量为120 g·kg-1体重的银杏叶复方制剂进行MTD试验，7 d后处死观察其病理变化。②分别用形态学观察法和MTT法进行细胞毒性试验，用细胞维持液将100 g·L-1银杏叶复方制剂原液稀释成2-n（n为1-8）8个稀释度，测试银杏叶复方制剂在鸡胚成纤维细胞（CEF）的体外培养中的最大安全质量浓度（TC0）。结果表明，银杏叶复方制剂LD50〉40 g·kg-1体重，MTD〉120 g·kg-1体重，小鼠无一例出现死亡，剖检未见明显肉眼可见的病理变化，说明药物毒性很小，临床使用安全。在形态学观察法中，银杏叶复方制剂在高浓度时未表现细胞毒性，在MTT法中银杏叶复方制剂对CEF的最大安全质量浓度为0.78 g·L-1。

Study the acute toxicity in mice and cytotoxicities of Ginkgo biloba compound to make sure the safety of clinical application. ① The half lethal dose （LD50） and the maximum tolerance dose （MTD） were the indexes in the acute toxicity test. The mice were given Ginkgo biloba compound through intragastric administration at the dose of 40 g·kg-1·W. The mice were still alive after 1 week, then they were given the compound at the dose of 120 g·kg-1·W. everyday at the condition of the most tolerant dose. After 1 week, the mice were killed to observe the pathological change.②The cytotoxicity tests were carried out by the morphology observation and the MTT assay. 100 g·L-1 of Ginkgo biloba compound was diluted into 2-n （n is from 1 to 8） with the cell maintenance medium, to test the most safety concentration（TC0） of Ginkgo biloba compound on chick embryo fibroblast （CEF）. The results showed that the acute toxicity of Ginkgo biloba compound was very low. The LD50 and MTD was above 40 g·kg-1·W. and 120 g·kg-1·W., respectively. The mice were still alive and had no pathological change.According to the toxicity level standard, the short period use of compound was safe. Ginkgo biloba compound did not exhibit cytotoxicity at high concentrations in the morphology observation. The TC0 of Ginkgo biloba compound was 0.78 g·L-1 in the MTT assay.