大孔吸附树脂纯化蒺藜呋甾皂苷和总黄酮工艺研究

Purification Technology of Furostanol Saponins and Total Flavonoids from Tribulus terrestris L. by Macroporous Resin

目的:研究大孔吸附树脂纯化蒺藜呋甾皂苷、总黄酮的工艺条件。方法:以蒺藜呋甾皂苷、总黄酮含量及其转移率为指标,采用单因素试验法,优选树脂富集纯化蒺藜呋甾皂苷和总黄酮的最佳工艺。结果:纯化最优工艺条件:上样量为0.4g/mL,树脂径高比为1∶8,4BV水以2mL/min流速洗脱,分别用5BV50%乙醇、2BV/h 70%乙醇洗脱。D101树脂纯化后蒺藜呋甾皂苷纯度由22.59%提高到53.54%,增加了1.37倍;总黄酮的纯度由12.46%提高到32.11%,增加了1.58倍。结论:D101大孔吸附树脂可用于蒺藜呋甾皂苷和总黄酮的纯化。

Objective ： To discuss purification technology conditions of furostanol saponins and total flavonoids from Tribulus terres- tris L. by macroporous resin. Methods： With the the content of furostanol saponins and total flavonoids as indexes, purification technology conditions were optimized by single factor experiment. Results：Optimum purification conditions were as follow： concen- tration of sample liquid was 0.4g/ml, ratio of diameter to height of macroporous resin column was 1 ： 8, them were eluted by 4BV water at 2mL/min, 5BV 50% and 70% ethanol at 2BV/h respectively. The purity of furostanol saponins reached 53.54%, which was two times higher than that before purification. And the purity of total flavonoids reached 32.11% ,which was two times higher than that before purification. Conclusion：D101 Macroporous Resin is suitable for the purification of furostanol saponins and total flavonoids from Tribulus terrestris L.