湿注骨节膝骨关节炎关节液CS和KS分析及临床意义

Analysis and Clinical Significance of Keratin Sulfate and Chondroitin Sulfate in Synoivial Fluid of Knee Osteoarthritis of Dampness Pathogen Stagnating in Joint

目的:探讨湿注骨节膝骨关节炎关节液中硫酸软骨素(CS)和硫酸角质素(KS)含量与关节软骨损伤程度的关系。方法:2011年5月—2012年12月收集膝骨关节炎病例63例(67膝),Kellgren和Lawrecne分级,采用ELISA法用酶标仪测定CS和KS含量,与健康对照组比较,分析KOA软骨损伤Kellgren和Lawrecne分级与CS和KS含量的相关性。结果:Kellgren和Lawrecne分级0级与1级CS含量分别显著高于2、3、4级(P<0.05),1级与0级KS含量分别显著高于3级和4级(P<0.05),4级KS/CS显著高于0、1、2、3级(P<0.05)。结论:湿注骨节KOA关节液KS和CS含量的变化可应用于关节软骨明显损伤与否的评估,CS/KS增高能够提示关节软骨的损伤严重,已经波及软骨下骨质。

Objective ： To investigate the relationship between content of ehondroitin sulfate （ CS ） and keratan sulfate （ KS ） and degree of articular cartilage damage in knee osteoarthritis synovial fluid. Methods： From May 2011 to December 2012, we collected 63 cases of knee osteoarthritis （ 67 knees ）. Kellgren and Lawrecne grading, CS and KS levels were measured by ELISA using a mieroplate reader. Compared with the healthy control group, we analyzed the relevance between Kellgren and Lawrecne classification and content of KS and CS in KOA cartilage damage. Results ： Accroding to Kellgren and Lawrecne classification, the contents of CS in graded 0 and 1 were significantly higher than 2,3,4 level （ P〈0.05 ）. KS in graded 0 and 1 was significantly higher than 3,4 level （ P〈0.05 ）. KS / CS in graded 4 was significantly higher than 0, 1,2,3 level （ P〈0.05 ）. Conclusions ： The content of changes in KS and CS can be used to assess articular cartilage obvious damage or not. The increase of CS/KS can prompt severe articular cartilage damage, and has spread subchondral bone.