黄连、黄芩药对提取物中黄芩苷和盐酸小檗碱药代动力学研究

Study on Pharmacokinetics in Combination Extracts of Coptidis Rhizoma and Scutellariae Radix

目的：建立高效液相色谱法测定大鼠血浆中黄芩苷和盐酸小檗碱,进行药代动力学研究。方法：以紫草酸为内标,采用Agilent C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.3%磷酸三乙胺（22∶78）,流速1.0 mL·min-1,检测波长278 nm,进样体积20μL。结果：黄芩苷和盐酸小檗碱呈良好线性关系;回收率分别为82.1%~87.1%、80.1%~87.5%,日内、日间精密度的RSD均小于10%。结论：为黄芩苷、盐酸小檗碱的药代动力学研究提供方法学依据。

Abstract： Objective： To develop an HPLC method for the determination of baicalin and berberine hydrochloride in rat plasma for the subsequent pharmacokinetic study. Methods ： Purple oxalic acid as internal standard, Agilent C18 Chromatographic column （4.6 mm× 250 mm, 5 μ m ）with the column temperature of 30 ℃ was used. The mobile phase consisted of three-0.3% phosphate triethylamine （ 22： 78 ） with flow rate of 1.0 mL · min-i, and the detection wavelength was set at 278 nm and the injection volume was 20 μ L. Results ： The developed method had a good linearity in the ranges. Low and high recoveries of the 2 mass concentration were 82.1% - 87.1%, 80.1% - 87.5%. Days, inter-day precision of RSD was less than 10%. Conclusion ： Pharmacokinetic studies provide methodological basis for baicalin and berberine hydrochloride.