Plackett-Burman联用Box-Behnken效应面设计优选夜交藤总黄酮提取工艺

Optimization Extraction of Total Flavones from Polygoni multiflori Caulis Based on Plackett-Burman Combined with Box-Behnken response Surface Methodology

目的:运用Plackett-Burman实验设计联用Box-Behnken效应面设计,考察各影响因素显著性,筛选夜交藤总黄酮提取的最佳工艺。方法:运用Plackett-Burman设计筛选主要影响因素,Box-Behnken效应面设计优选夜交藤黄酮提取工艺。乙醇浓度、料液比、超声时间作为自变量,黄酮含量为因变量,通过对其响应面的回归拟合,确定夜交藤黄酮提取最佳工艺,并进行预测分析。结果:黄酮的最佳提取工艺为:超声功率为400 W,乙醇浓度85%,料液比为1∶25,提取温度40℃,提取2次,20 min/次。结论:Plackett-Burman联用Box-Behnken效应面设计优选了夜交藤总黄酮的提取工艺,该方法简便,精度更高、重现性好、预测性强。

Objective： To optimize the process of extracting effective constituents from Polygoni muhiflori Caulis by Plaekett-Burman combined with Box-Behnken response surface methodology. Methods： In Plackett-Burman combined with Box-Behnken response surface design, independent variables were concentration of ethanol, liquid ratio, ultrasonic time. Dependent variables were contents of total flavones. Results：The optimization extraction of total flavones was 400W ultrasonic power, 85% ethanol,25 fold of solvent and 2 times for ultrasonic extraction at the 40 ℃, 20 minutes each time. Conclution ： The optimal process is simple and more convenient for extracting the total flavones from Polygoni muhiflori Caulis with higher precision and predictability.