脑血宁注射液中牡丹皮药材HPLC指纹图谱研究

Fingerprint of Moutan in Naoxuening Injection by HPLC

目的:建立脑血宁注射液组方中牡丹皮的指纹图谱。方法:使用Diamonsil C18柱(4.6 mm×150 mm,5μm);以乙腈-0.1%磷酸(三乙胺调pH至3.0),为流动相,梯度洗脱,在238 nm波长下检测。结果:建立了牡丹皮药材的高效液相(HPLC)指纹图谱,确定牡丹皮药材有14个共有峰,不同产地、批次的牡丹皮的共有峰的相对保留时间和相对保留面积稳定,但是不同产地的牡丹皮中的各组分的绝对峰面积存在一定差异,以丹皮酚计,安徽产地的牡丹皮的含量最高。结论:该方法准确、可靠,为加强脑血宁注射液原料药的质量控制,保证脑血宁注射液的质量提供了依据。

Objective ： To establish the HPLC fingerprints of Moutan in Naoxuening Injection. Methods ： The chromatographic separation was performed on a Diamonsil Cls column （ 4.6 mm×150 mm, 5 μm ）by gradient eluent using the mobile phase consisting of acetonitrile-0.1% phosphoric acid （ pH was adjusted to 3.0 by triethylamine ）. The detection wavelength was set at 238 nm. Results ： The fingerprint of Moutan was established, and fourteen common peaks in the chromatogram were marked with the parameter according to the technical requirements of the fingerprints of Chinese traditional medicine. The ratio of common peak areas was comparable in the fingerprints of Moutan from different places and different batches. However, the content of paeonol （ a major active ingredient of Moutan ） was different in Moutan from different places. Conclusion ： The method was accurate and reliable, and provided a basis for controlling the quality of Naoxuening Injection.