细胞角蛋白19mRNA转录与蛋白表达在结肠癌术后感染患者外周血中的应用

Significance of cytokeratin 19mRNA transcription and protein expression in peripheral blood of colon cancer patients with postoperative infections

目的探讨细胞角蛋白19mRNA转录与蛋白表达在结肠癌术后感染患者外周血中的应用及意义,以期提高临床诊断水平。方法随机选取2012年1月-2013年6月50例结肠癌术后感染患者为研究对象,另选取同时期的50名健康人员及50例结肠良性病变患者各作为对照,使用酶联免疫吸附检测细胞角蛋白19(CK19)在外周血中的表达水平。结果 CK19在结肠癌术后感染中表达阳性41例,阳性率为82.0%,与结肠良性病变和健康人群组比较差异有统计学意义(P<0.05);且CK19表达和结肠癌术后感染在肿瘤类型、分化程度、淋巴结转移和Dukes分期上的分型之间比较差异有统计学意义(P<0.05)。结论 CK19mRNA能早期发现结肠癌病变情况。

OBJECTIVE To explore the significance of cytokeratin 19 mRNA transcription and protein expression in the peripheral blood of the colon cancer patients with postoperative infections so as to improve the level of clinical diagnosis. METHODS From Jan 2012 to Jun 2013, a total of 50 colon cancer patients with postoperative infections were randomly recruited in the study, meanwhile, 50 healthy people and 50 patients with benign colon lesions were chosen as controls, then the enzyme-linked immunosorbent assay was performed to determine the level of expression of cytokeratin 19 in the peripheral blood. RESULTS Totally 41 patients were tested positive for expression of cytokeratin 19, with the positive rate of 82.0%, as compared with the patients with benign colon lesions or the healthy people, there was significant difference （P〈0.05）; the differences in the expression of cytokeratin 19 and the incidence of postoperative infections between the subjects with different types of tumors, degree of differentiation, lymph node metastasis, or genotypes in Dukes stage were significant （P〈0.05）. CONCLUSION The CK19 mRNA can detect the colon cancer lesions in early stage.