超临界CO_2流体萃取技术降低哈蟆油中雌激素的工艺优选

Optimization of Supercritical CO_2 Fluid Extraction Process for Deduction of Estrogen in Ranae Oviductus

目的:优选超临界CO2流体萃取技术降低哈蟆油中雌激素含量的工艺条件。方法:采用放射免疫法测定雌激素(雌二醇、雌三醇)含量,以哈蟆油中雌激素的平均去除率为指标,通过正交试验考察萃取温度、萃取时间、萃取压力、夹带剂用量对雌激素CO2超临界萃取工艺的影响。结果:最佳萃取工艺条件为温度50℃,压力30 MPa,乙醇夹带剂用量35 mL,萃取时间4 h;雌激素平均除去率32.12%,1-甲基海因剩余率88.7%。结论:优选的CO2超临界萃取工艺稳定可行,能有效降低哈蟆油中雌激素含量且基本保留了哈蟆油润肺止咳的有效成分。

Objective：To optimize supercritical CO2 fluid extraction process for reducing the content of estrogen in Ranae Oviductus.Method：The content of estrogen （estradiol and estriol） was determined by radioimmunoassay,taking average removal rate of estrogen in Ranae Oviductus as index,orthogonal design was adopted to optimize supercritical CO2 fluid extraction process with entrainer dosage,extraction temperature,time and pressure as factors.Result：Optimum process conditions were as follows：extracted 4 h at temperature of 50 ℃ and pressure of 30 MPa,with 35 mL of ethanol as entrainer; average removal rate of estrogen was 32.12％,surplus ratio of 1-methyl hydantoin was 88.7％.Conclusion：This optimized supercritical CO2 extraction process is stable and feasible,which has a good effect on reducing the content of estrogen in Ranae Oviductus and basically retains active ingredients for moistening lung to arrest cough.