响应曲面法优化伸筋草总生物碱的微波辅助提取工艺

Optimization of Microwave-assisted Extraction Technology for Total Alkaloids from Lycopodii Herba by Response Surface Methodology

目的:优化微波辅助提取伸筋草总生物碱的工艺条件。方法:采用酸性染料比色法测定总生物碱含量,以石松灵碱为指标成分。在单因素试验基础上,以总生物碱提取率为评价指标,选取液料比、提取温度和提取时间3个因素进行BoxBehnken中心组合设计,采用响应曲面法优化伸筋草总生物碱的微波辅助提取工艺。结果:伸筋草总生物碱的最佳提取工艺为加17倍量甲醇-水-盐酸(85∶15∶1)于72℃提取8 min;总生物碱提取率4.092 mg·g-1,与预测值4.070 mg·g-1的偏差较小。结论:与传统提取工艺相比,优选的微波辅助提取工艺具有时间短、提取率高、能量消耗低等优点。

Objective：To optimize microwave-assisted extraction process conditions of total alkaloids from Lycopodii Herba.Method：Acid dye colorimetry was employed to determine the content of total alkaloids with lycodoline as index component.Based on single factor tests,effects of liquid-solid ratio,extraction temperature and time on yield of total alkaloids were investigated with a three-factor and three-level Box-Behnken central composite design,microwave-assisted extraction conditions of total alkaloids from Lycopodii Herba was optimized by response surface methodology and compared with traditional extraction technique.Result：Optimum conditions were as follows：extracted 8 min with 17 times the amount of methanol-water-hydrochloric acid （85∶15∶1） at 72 ℃ ;yield of total alkaloids was 4.092 mg ·g-1 under these conditions,which was consistent with the theoretic value of 4.070 mg · g-1 Conclusion：Microwave-assisted extraction process has many advantages over conventional methods,such as time saving,high extraction ratio and low solvent consumption.