摘要
目的:优化微波辅助提取伸筋草总生物碱的工艺条件。方法:采用酸性染料比色法测定总生物碱含量,以石松灵碱为指标成分。在单因素试验基础上,以总生物碱提取率为评价指标,选取液料比、提取温度和提取时间3个因素进行BoxBehnken中心组合设计,采用响应曲面法优化伸筋草总生物碱的微波辅助提取工艺。结果:伸筋草总生物碱的最佳提取工艺为加17倍量甲醇-水-盐酸(85∶15∶1)于72℃提取8 min;总生物碱提取率4.092 mg·g-1,与预测值4.070 mg·g-1的偏差较小。结论:与传统提取工艺相比,优选的微波辅助提取工艺具有时间短、提取率高、能量消耗低等优点。
Objective:To optimize microwave-assisted extraction process conditions of total alkaloids from Lycopodii Herba.Method:Acid dye colorimetry was employed to determine the content of total alkaloids with lycodoline as index component.Based on single factor tests,effects of liquid-solid ratio,extraction temperature and time on yield of total alkaloids were investigated with a three-factor and three-level Box-Behnken central composite design,microwave-assisted extraction conditions of total alkaloids from Lycopodii Herba was optimized by response surface methodology and compared with traditional extraction technique.Result:Optimum conditions were as follows:extracted 8 min with 17 times the amount of methanol-water-hydrochloric acid (85∶15∶1) at 72 ℃ ;yield of total alkaloids was 4.092 mg ·g-1 under these conditions,which was consistent with the theoretic value of 4.070 mg · g-1 Conclusion:Microwave-assisted extraction process has many advantages over conventional methods,such as time saving,high extraction ratio and low solvent consumption.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第18期34-37,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
伸筋草
微波辅助法
总生物碱
响应曲面法
石松灵碱
Lycopodii Herba
microwave-assisted extraction
total alkaloids
response surface methodology
lycodoline