摘要
目的:优选酸荔枝核中抗乙肝黄酮类化合物的提取工艺。方法:以芦丁为对照品,采用UV测定总黄酮含量。以总黄酮提取率为指标,温度、粉碎度、乙醇体积分数、料液比、提取时间、搅拌速度为自变量,在单因素试验基础上,通过Plackett-Burman试验筛选显著影响因子,利用Box-Behnken设计考察各自变量与响应值之间的关系,模拟得到二次多项式回归方程的预测模型,确定最佳提取工艺。结果:粉碎度和乙醇体积分数是影响荔枝核中总黄酮提取效率的显著因子,最佳提取工艺条件为粉碎度120目,加14倍量75%乙醇于60℃提取2 h,搅拌速度90 r·min-1;总黄酮平均提取率17.49%(RSD0.63%)。结论:优选的提取工艺稳定可行,适用于酸荔枝核中抗乙肝总黄酮类化合物的工业化生产。
Objective:To optimize extracting process of total flavonoids from acid Litchi Semen.Method:Taking rutin as standard,the content of total flavonoids was determined by UV.With yield of total flavonoids as index,on the basis of single factor tests,Box-Behnken and Plackett-Burman tests were adopted to optimize extraction process by taking temperature,grinding degree,ethanol concentration,liquid-solid ratio,extracting time and stirring speed as factors.Result:Grinding degree and ethanol concentration were significant factors,optimum extraction technology was as following:grinding degree of 120 mesh,extracted 2 h with 14 times the amount of 75% ethanol at 60 ℃,stirring speed of 90 r ·min-1 Under these conditions,average extraction rate of total flavonoids was 17.49% with RSD of 0.63%.Conclusion:This optimized technology is stable and feasible,which is suitable for industrial production of anti-hepatitis B flavonoids from acid Litchi Semen.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第18期41-44,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西教育厅优秀人才计划项目(20124147)
广西高校大学生创新计划项目(QJCX201335)
广西教育厅科研项目(201010LX373
201106LX441
200103YB114)
关键词
响应曲面法
酸荔枝核
抗乙肝成分
总黄酮
response surface methodology
acid Litchi Semen
anti-hepatitis B component
total flavonoids