响应曲面法优化野生酸荔枝核中抗乙肝成分的提取工艺

Optimization of Extraction Process for Anti Hepatitis B Consituents from Acid Litchi Semen by Response Surface Methodology

目的:优选酸荔枝核中抗乙肝黄酮类化合物的提取工艺。方法:以芦丁为对照品,采用UV测定总黄酮含量。以总黄酮提取率为指标,温度、粉碎度、乙醇体积分数、料液比、提取时间、搅拌速度为自变量,在单因素试验基础上,通过Plackett-Burman试验筛选显著影响因子,利用Box-Behnken设计考察各自变量与响应值之间的关系,模拟得到二次多项式回归方程的预测模型,确定最佳提取工艺。结果:粉碎度和乙醇体积分数是影响荔枝核中总黄酮提取效率的显著因子,最佳提取工艺条件为粉碎度120目,加14倍量75%乙醇于60℃提取2 h,搅拌速度90 r·min-1;总黄酮平均提取率17.49%(RSD0.63%)。结论:优选的提取工艺稳定可行,适用于酸荔枝核中抗乙肝总黄酮类化合物的工业化生产。

Objective：To optimize extracting process of total flavonoids from acid Litchi Semen.Method：Taking rutin as standard,the content of total flavonoids was determined by UV.With yield of total flavonoids as index,on the basis of single factor tests,Box-Behnken and Plackett-Burman tests were adopted to optimize extraction process by taking temperature,grinding degree,ethanol concentration,liquid-solid ratio,extracting time and stirring speed as factors.Result：Grinding degree and ethanol concentration were significant factors,optimum extraction technology was as following：grinding degree of 120 mesh,extracted 2 h with 14 times the amount of 75％ ethanol at 60 ℃,stirring speed of 90 r ·min-1 Under these conditions,average extraction rate of total flavonoids was 17.49％ with RSD of 0.63％.Conclusion：This optimized technology is stable and feasible,which is suitable for industrial production of anti-hepatitis B flavonoids from acid Litchi Semen.