黔产白及的化学成分

Chemical Constituents of Bletillae Rhizoma

目的:对黔产白及药材进行化学成分研究。方法:应用MCI、正相硅胶、Sephadex LH-20凝胶以及半制备型HPLC分离纯化,运用1H-NMR,13C-NMR,ESI-MS等现代波谱技术对化合物的结构进行鉴定。结果:从黔产白及中分离得到其中7个化合物,经波谱数据及理化性质鉴定为α-异苹果酸(1),4,4'-二羟基二苯基甲烷(2),留兰香木脂素B(3),gymnoside V(4),4,4'-二羟基苄基硫醚(5),gymnoside IX(6)以及gymnoside X(7)。结论:7个化合物均为首次从该属植物中分离得到。

Objective：This investigation aimed to study the chemical constituents of Bletillae Rhizoma.Method：Normal phase silica gel,MCI,and Sephadex LH-20 column chromatographies and semi-prep HPLC were used to isolate and purify the chemical constituents.Their structures were identified by spectroscopic methods,including 1H-NMR,13C-NMR and ESI-MS.Result：Seven compounds were isolated and identified as α-isobutylmalic acid （1）,4,4＇-dihydro xydiphenylmethane （2）,spicatolignan B （3）,gymnosides V （4）,4,4＇-dihydroxybenzyl sulfide （5）,gymnoside Ⅸ （6） and gymnoside X （7）.Conclusion：All compounds were isolated from Bletilla for the first time.