经络舒宁胶囊有效部位组方对大鼠血糖及糖化血红蛋白的影响

Effect of Jingluo Shuning Capsule Effective Parts Combinations on Blood Sugar and Glycosylated Hemoglobin in Rats

目的:研究经络舒宁有效部位的不同剂量、两两配伍、不同配比对大鼠血糖和糖化血红蛋白的影响。方法:采用链尿佐菌素(48 mg·kg-1)1次性ip的方法建立糖尿病大鼠模型,将大鼠随机分成空白组(健康大鼠给予等容量生理盐水)、模型组(等量生理盐水)、阳性药物组(盐酸二甲双胍230 mg·kg-1)、经络舒宁有效部位高、中、低组(34.4,68.7,137.4 mg·kg-1)以及按三因素(丹参、三七、水飞蓟素)三水平(高、中、低剂量)正交设计配比的9个不同组合。连续灌胃28 d,分别于第2,5,10,15,25,29天测定大鼠的空腹血糖;于第29天测定糖化血红蛋白含量。结果:连续灌胃28 d后,以监测血糖的变化和第29天糖化血红蛋白含量的测定为指标,首先比较有效部位组合高、中、低剂量对糖尿病大鼠的影响,确定了中剂量组为最佳剂量(与模型组比有极显著差异,P<0.01);在此基础上,最佳剂量组与两两配伍的不同组合进行了组间比较,结果表明最佳剂量组控制血糖和糖化血红蛋白的效果最明显(与模型组比有极显著差异,P<0.01);最后通过3种有效部位不同剂量的配比分别测定指标值,组间比较表明组合5的降糖作用最佳。结论:经络舒宁有效部位组合5与模型组相比有显著性差异,能有效降低血糖,控制糖尿病症状,为临床上用于糖尿病神经病变提供了实验依据。

Objective：To study blood sugar and glycosylated hemoglobin of different doses and different compatibility of effective parts from Jingluo Shuning capsule.Method：A single intraperitoneal injection of streptozotocin （48 mg ·kg-1） was used to induce diabetic rats.The modling rats were randomly divided into control group,modelgroup,Jingluo Shuning groups （34.4,68.7,137.4 mg · kg-1） and the 9 compatility groups according to the combination of three factors and three levels orthogonal design ratio.The oral administration lasted for 28 days.The fasting blood glucose levels and glycosylated hemoglobin （GHb） were measured.Result：The middle dose group had significant effect.On this basis,the different dosage group and two two compatibility were compared,the results showed the optimal dose.Finally,in the formula of the three effective parts the combination 5,indicated the best effect.Conclusion：In Jingluo Shuning effective parts combination 5 has the most significant effect on the blood sugar and GHb.