前列泌尿栓抗菌和抗炎作用研究

Antibiosis and Antiphlogosis Effects of Qianlie Miniao Shuan

目的:研究前列泌尿栓对急性前列腺炎的抗菌和抗炎作用。方法:60只小鼠随机分为6组:空白组,模型组,阳性药野菊花栓组,前列泌尿栓高、中、低剂量组,前列泌尿栓组按照0.25,0.19,0.13 g·kg-1剂量直肠给药,3 d后ip临床分离变形杆菌菌液或临床分离大肠埃希菌菌液造成体内细菌感染模型,感染24 h后,再连续给药5 d,观察2周,观察前列泌尿栓对小鼠死亡的保护作用;取前列泌尿栓溶液与血清肉汤培养基混合,制成不同药物质量浓度(250.8,188.1,125.4,62.7,31.4g·L-1)的平皿,每个浓度做4个平皿,加入稀释好的菌液,37℃培养48 h,观察最小杀菌浓度(MBC)和最小抑菌浓度(MIC),观察前列泌尿栓对急性前列腺炎主要致病菌的抑菌和杀菌作用;通过大鼠感染大肠埃希菌或变形杆菌造成急性前列腺炎模型的作用,观察前列泌尿栓的消炎作用。结果:感染变形杆菌或大肠埃希菌的小鼠在给予前列泌尿栓后3个剂量组的死亡率均低于模型组(P<0.05);对金黄色葡萄球菌、沙门菌、铜绿假单胞菌、大肠埃希菌、变形杆菌(临床分离)、大肠埃希菌(临床分离)的MBC分别为62.7,125.4,250.8,188.1,250.8,188.1 g·L-1,MIC分别为31.4,62.7,188.1,125.4,188.1,125.4 g·L-1;对变形杆菌或大肠埃希菌造成的急性前列腺炎模型在给予前列泌尿栓后前列腺脏器系数均明显小于模型组,结果有统计学意义(P<0.05),病理切片显示3个剂量组组织恢复均好于模型组,明显降低大鼠前列腺组织炎性细胞浸润(P<0.05)。结论:前列泌尿栓对急性前列腺炎有良好的抗菌和消炎作用。

Objective：To study the antibiosis and antiphlogosiseffects of Qianlie Miniao Shuan on acute prostatitis.Method：Sixty mice were randomly divided into six groups：blank group,model group,positive group and high,middle,low dosage groups of Qianlie Miniao Shuan （0.25,0.19,0.13 g ·kg-1）.The model was induced by bacterial infection of Proteus （clinical isolates） or Escherichia coli （clinical isolates） in vivo by intraperitoneal injection after three days.The drugs were given for 5 days after mice infected 24 hours.The protective effect of mouse death was observed by the mouse model infected by E.coli or Bacillus proteus in vivo Qianlie Miniao Shuan dosing.The Petri dishes mixed qianlie miniao shuan solution and serum broth were made of different drug concentration （250.8,188.1,125.4,62.7,31.4 g · L-1）,each concentration was 4 Petri dishes.After added broth dilution,the Petri dishes were cultured at 37 ℃ for 48 h,and then we observed minimal bactericidal concentration （MBC） and minimal inhibitory concentration （MIC）.The antibacterial and bactericidal effect of Qianlie Miniao Shuan on the main pathogens of acute prostatitis was observed by the antibacterial test in vitro.The anti-inflammatory effect of Qianlie Miniao Shuan was observed by the acute prostatitis model in rats infected by E.coli or B.proteus.Result：The mortality rates of the mice infected Proteus infection or E.coli given Qianlie Miniao Shuan in the three dosages groups were lower than the model group （P ＜ 0.05）.The MBC of Staphylococcus aureus,Salmonella,Pseudomonas aeruginosa,E.coli,Proteus （clinical isolates） and E.coli （clinical isolates） were 62.7,125.4,250.8,188.1,250.8,188.1 g·L-1,The MIC of S.aureus,Salmonella,P.aeruginosa,E.coli,Proteus （clinical isolates） and E.coli （clinical isolates） were 31.4,62.7,188.1,125.4,188.1,125.4 g ·L-1 The prostate organ coefficient of acute prostatitis model caused by Proteus or E.coli gived qianlie miniao shuan were less than the model group,the difference had statistical significance （P ＜ 0.05）.The pathological results showed the tissue renewing in the three dose groups was better than that in the model group.The Qianlie Miniao Shuan could significantly reduce the inflammatory infiltration of prostate tissue in rats （P ＜ 0.05）.Conclusion：Qianlie Miniao Shuan for acute prostatitis has good antibacterial and antiinflammatory effects.