抑瘤素M在糖尿病小鼠肾组织中的表达

Expression of oncostatin M in kidney of diabetic mice

目的探讨抑瘤素M(oncostatin M,OSM)在糖尿病小鼠肾组织中的表达。方法腹腔注射链脲佐菌素(Streptozotocin,STZ;150 mg/kg)诱发糖尿病小鼠模型,分别于成模后4、8和12周收集肾组织标本进行以下检测:免疫组织化学和酶联免疫吸附实验检测肾组织中OSM的表达;马松染色观察肾小管间质纤维化程度。结果与对照组相比,糖尿病小鼠肾组织中OSM的表达明显高于对照组增多,且其表达与肾小管间质纤维化程度呈正相关(r=0.946,P<0.01)。结论 OSM在糖尿病小鼠肾组织中的表达升高并可能参与了肾小管间质纤维化的进展。

[Objective ] To investigate the expression of oncostatin M （OSM） in the renal cortex of diabetic mice. [Method] Male CD-1 mice were randomly divid~~d into two groups： control group （N） and diabetic group （DM）. The mice of diabetic group received a single intraperitoneal injection of STZ at a dose of 150 mg/kg body weight. The expression of OSM was detected by the methods c f immunohistochemistry and ELISA. Masson staining was used for calculating the renal interstitial fibrosis area（the percentage of the blue stain to the whole area）. [ Results ] Compared with the control mice, the OSM expression increased in mice with diabetes. The expression level of OSM is positively correlated with the degree of tubulointerstitial fibrosis （r =0.946, P 〈0.01）. [ Conclusion] In diabetic mice, the OSM expression is increased, which maybe involved in the progression of tubulointerstitial fibrosis.