热休克蛋白70在帕金森症患者外周血单核细胞中的表达及意义

Reduced expression of the chaperone-mediated autophagy carrier HSC70 protein in lymphomonocytes of Parkinson's diseasepatients

目的探讨PD患者外周血细胞中监测CMA活性的生物标志物。方法 53例散发性PD患者和53名正常人纳入该次研究。检测PD患者和正常人外周血单核细胞中HSC70,lamp2A和MEF2D表达情况。蛋白和基因表达水平分别由Western blot和real-time PCR检测。结果在基础状态下或血清剥脱引起的自噬状态下,PD患者外周血单核细胞HSC70表达显著下降。lamp2A和MEF2D在PD患者和健康人群中表达无差异。HSC70,lamp2A和MEF2D表达对PD患者临床特征无影响。结论 HSC70在PD患者外周血单核细胞中表达显著下降。

[ Objective ] To identify new promising candidates for monitoring CMA activity in peripheral cells obtained from PD patients. [Methods] The expression of HSC70 and lamp2A was assessed in peripheral blood mononuclear cells （PBMC） of patients with sporadic PD compared with healthy subjects. The expression of myocyte enhancer factor 2D （MEF2D）, a transcriptional factor implicated in neuronal survival and specific substrate of CMA, was also evaluated. Protein and gene expression wereassessed by Western blot and real-time PCR, respectively, in PBMC obtained from 53 sporadic PD patients and 53 healthy subjects. [ Results ] A significant reduction of HSC70 levels was observed in PBMC of PD patients , both under basal conditions and after autophagy induction obtained with serum deprivation. No difference emerged in lamp2A and MEF2D expression between patients and controls. No influence of the clinical characteristics of patients emerged on HSC70, lamp2A and MEF2D expression. [ Conclu- sion ] A systemic HSC70 is reduced in PD patients.