摘要
[目的]确定荞麦种子发芽的适宜甲基磺酸乙酯(EMS)诱变剂量,为进一步获得荞麦优良性状并培育符合EMS诱变育种需求的新品种打下坚实的基础.[方法]以山西省当地的苦荞[Fagopyrum tataricum(L.)Gaertn]品种晋荞麦4号种子为材料,用不同浓度梯度(0.3%、0.5%、0.7%、1.0%、1.5%、1.7%)EMS诱变处理不同时间(4、8、12 h),分析苦荞种子发芽率和发芽势.[结果]随着EMS诱变剂浓度的增加,晋荞麦4号种子发芽势、相对发芽势和发芽率、相对发芽率均呈下降趋势,当EMS浓度为0.3% ~1.0%时与对照没有显著差异,当EMS浓度为1.5%和1.7%时与对照差异极显著.EMS处理4、8h对晋荞麦4号种子发芽率、相对发芽率都没有大的影响,当处理时间为12 h时,与对照有显著差异;对于发芽势和相对发芽势,这3个处理时间没有显著差异.[结论]EMS诱变苦养的半致死的适宜浓度为1.7%,适宜的处理时间为12 h.
[ Objective ] The aim was to confirm on the suitable EMS dosage of tarary buckwheat seed gemination,and further lay a solid founda- tion for obtaining excellent traits of buckwheat and breeding the new varieties meeting EMS mutagenesis breeding. [ Method] The seeds of Jinqiao- mai No. 4 ,a Shanxi local tarary buckwheat variety,were mutagenized by different concentration(0.3% ,0.5% ,0. 7% ,1.0% ,1.5% ,1.7% ) EMS for 4,8 and 12 h,to analyze their gemination rate and gemination vigor. [ Result ] With the increase of EMS mutagenic agent concentration, the gemination vigor,relative gemination vigor, gemination rate and relative gemination rate of Jinqiaomai No. 4 all showed the decrease trend, they had no significant difference with control when EMS concentration was 0.3% - 1.0%, while had very significant difference with control when EMS concentration was 1.5% and 1.7%. Using EMS mutagenic agent to mutagenize Jinqiaomai No.4 seeds for4 and 8 h had no big effects on its gemination rate and relative gemination rate,while when the treatment time was 12 h,they had significant difference with control; For gemination vigor and relative gemination vigor,this three treatment time had no significant difference. [ Conclusion] The suitable concentration and time of the median lethal dose of EMS mutagenizing tarary buckwheat seed is 1.7% and 12 h,resp.
出处
《安徽农业科学》
CAS
2014年第26期8938-8939,共2页
Journal of Anhui Agricultural Sciences
基金
山西省农业科学院玉米研究所攻关项目(SGG2014-3)
关键词
苦荞
发芽率
发芽势
Tarary buckwheat
EMS
Germination rate
Germination vigor