不同饲养模式对杂交鹿血清MBL抗菌活性的影响

Effect of Different Feeding Modes on Serum of MBL Antibacterial Activity in Hybrid Deer

[目的]探索不同饲养模式对杂交鹿血清中MBL抗菌活性的影响。[方法]采用平板计数法研究双阳梅花鹿和清原马鹿杂交F1代MBL的抗菌活性,探讨MBL作为补体功能候选蛋白的可行性,分析MBL抗化脓感染创伤致病菌的活性。[结果]放养模式试验组抗金黄色葡萄球菌活性和抗大肠杆菌活性都显著高于圈养模式试验组、D-甘露糖试验组和N-乙酰基-D-葡萄糖胺试验组(P<0.05)。放养模式试验组抗金黄色葡萄球菌活性和抗大肠杆菌活性对动物机体的保护作用具有显著的生物学意义。[结论]该研究可为杂交一代肉用鹿的抗病高产育种工作提供一定的理论依据和基础资料。

[ Objective] The research aimed to the effects of different feeding modes on the antibacterial activity of MBL in the serum of hybrid- ized deer. [ Method] The antibacterial activity of MBL in hybridized F1 generation of Shuangyang Deer and Qingyuan Wapiti was determined by using plate counting method. The feasibility of taking MBL as functional candidate protein of complement was discussed. The antibacterial activity of MBL against pathogens caused by pyogenic infection and wound was analyzed. [ Result ] The antibacterial activities of MBL to Staphylococcus aureus and Escherichia coli in grazing mode group were significantly higher than those in captive mode group, D-mannose group and N-acetyl -D-glucosamine group（P 〈0.05）. The antibacterial activities to S. aureus and E. coli in grazing model group were of significant biological meaning for protecting the animal bodies. [ Conclusion] The research could provide theoretical basis and basic data for disease-re- sistant and high-yield breeding of hybridized meat deer.