人肺癌组织中MCPH1基因过表达后协同化疗药物抑制肺癌A549细胞的增殖

Detection of MCPH1 mRNA expression in lung cancer and the effect of MCPH1 overexpression synergistic with chemotherapeutic drugs on tumor inhibition rate of A549 cells

目的检测人肺癌组织中MCPH1基因表达,并用MCPH1真核过表达载体,检测其协同化疗药物对人肺癌细胞A549的影响。方法用real-time PCR法检测肺癌标本中MCPH1基因相对表达量。用前期已构建的真核表达质粒pc DNA3.1(-)/MCPH1及对照空白质粒pc DNA3.1(-)转染A549细胞,实验分为3组:实验组(OVER)、空白对照组(W/O)、未处理组(NC)。然后分别用real-time PCR和Westen blot检测转染后细胞基因转录和蛋白表达。结果肺癌组织中MCPH1基因表达低于正常组织(P<0.05)。在肺癌A549细胞中分别加入阿霉素1.0μmol/m L、紫杉醇20μg/m L、顺铂0.1μg/m L,48 h后转染MCPH1组抑瘤率明显高于对照组及未处理组(P<0.05)。结论肺癌组织中MCPH1基因表达下调。MCPH1基因过表达后协同化疗药物抑瘤率明显升高。

Objective To detect MCPH1 mRNA expression in lung cancer and over expression of MCPH1 in A-549 cells with drugs. Methods MCPH1 mRNA expression in lung cancer was measured by real-time PCR. Eukaryotic expression plasmid pcDNA3.1 （ - ）/MCPH1 and Blank plasmid pcDNA3.1 （ - ） were transfected into A549 cells, and set three groups according to the different transfected plasmid ： Experimental group （ OVER）, Blank control group （W/O） ,Untreated （NC）. The MCPHI＇S mRNA and protein expression were detected by quantitative Real- Time PCR and Western bolt. Results MCPH1 gene expression in cancer tissues is lower than that of normal tissues （P 〈 0. 05）. In A549 cells, experimental groups were added respectively 1.0 μmol/mL ADM, 20μg/mLTAX, 0. 1μg/mL DDP,48 h later, The inhibition rate in experimental groups was significantly higher than that of control groups and the untreated groups （P 〈 0. 05 ）. Conclusions MCPH1 genes downregulated in lung cancer; the tumor inhibitory rate increased after MCPH1 gene overexpression concurrent chemotherapy drugs.