摘要
目的探究间碘苄胍(MIBG)对肝癌细胞HepG2增殖的影响及其可能机制。方法细胞免疫荧光法检测HepG2细胞中精氨酸特异性单腺苷二磷酸核糖基化转移酶1(ART1)的表达。MTT比色实验检测肝癌细胞HepG2的生存率。流式细胞计数法检测细胞周期。Westrenblot检测ART1、RhoA、c-myc和cyclinA1的表达。结果ART1在HepG2细胞有表达。MIBG对HepG2细胞增殖抑制呈剂量依赖性(P<0.05),主要是将细胞阻滞在S期(P<0.05)。MIBG能降低ART1、RhoA、c-myc和cyclinA1的表达水平(P<0.05)。结论MIBG通过影响RhoA信号通路,下调c-myc蛋白和cyclinA1蛋白的表达,将细胞阻滞在S期,而抑制HepG2细胞的增殖。
Objective To study the effects of MIBG（meta-iodobenzylguanidine） on proliferation of hepatoma carci- noma HepG2 cells and its possible mechanism. Methods The expression of ART1 in HepG2 cells was detected by cellular immunofluorescence method. The effect of different concentrations of MIBG on cell survival rate and cell cycle phases of HepG2 cells was measured by MTI＂ assay and flow cytometry. The expression of ART1, RhoA, c-myc and cyclinA1 were detected by Western blot. Results ART1 is found in HepG2 cells. The MIBG inhibits the growth of the HepG2 cells arrested in S phase, which is affected by different concentrations （ P 〈 0. 05 ）. The expression of the ART1, RhoA, c-myc and cyclinA1 decreases after using the MIBG （ P 〈 0. 05 ）. Conclusions MIBG, which down-regulated RhoA pathway and the downstream factors c-myc and cyclinA1, may inhibit the proliferation of HepG2 cells and arrest the cell cycles in S phase.
出处
《基础医学与临床》
CSCD
北大核心
2014年第9期1221-1225,共5页
Basic and Clinical Medicine
基金
高等学校博士学科点专项科研基金联合资助项目(20105503110009)
重庆市教委科学技术研究项目(KJ110322)
