P38-2G4蛋白在红系细胞分化过程中的动态表达分析

Analysis of dynamic expression of P38-2G4 during erythroid cells differentiation

目的 分析小鼠P38-2G4蛋白在红系细胞分化中的表达及功能,初步研究该蛋白是否参与红系分化过程.方法 制备E10.5 ～E16.5胎肝细胞、FVA细胞及诱导剂丁酸钠和六亚甲基二乙酰胺诱导不同时间的小鼠红白血病细胞MEL,用Western blot检测P38-2G4蛋白在上述3种细胞中的表达,同时以联苯胺染色法检测细胞分化的标志蛋白血红蛋白的表达.结果 在不同分化期的胎肝细胞中,P38-2G4蛋白的表达先升高后降低;在FVA细胞分化过程中,P38-2G4表达量同样先增加后减少;而在诱导剂诱导MEL细胞分化过程中,P38-2G4表达量呈下降趋势.结论 P38-2G4蛋白在不同红系分化组织中的差异性表达提示该蛋白参与了小鼠红系细胞的分化过程.

Objective Analysis of the expression and function of mouse P38-2G4 protein in the differentiation of er- ythroid cells, and to find whethere P38-2G4 is involved in the differentiation of erythroid. Methods The expres- sions of P38-2G4 in fetal liver from El0. 5 to El6. 5, FVA cells and MEL cells induced by SB and HMBA were de- tected by Western bolt. The hemoglobin as a marker protein of erythroid cell differentiation was detected by benzi- dine staining. Results The expression of P38-2G4 in fetal liver cells of different differentiating stages increased at first and then decreased; the expression of P38-2G4 during FVA cells differentiating had a same trend; but de- creased during inducing. Conclusions Differential expression of P38-2G4 in different erythroid differentiation shows that P38-2G4 is involved in mouse erythroid differentiation.