载神经生长因子纳米柔性脂质体制备及稳定性研究

Study on the preparation and stability of flexible nano-liposome loaded with nerve growth factor

目的研究载神经生长因子(NGF)纳米柔性脂质体的适宜制备的条件及稳定性。方法采用薄膜-超声法制备载NGF纳米柔性脂质体,ELISA法测定NGF含量,激光粒度分析仪检测粒径。结果制备载NGF纳米柔性脂质体的最适温度为30℃;pH值为7.0时,制备的纳米脂质体包封率最高;磷脂的适宜浓度为40 mg/ml;胆固醇与磷脂适宜比例为1∶4。室温保存30 d、4℃冰箱12个月,脂质体粒径无明显变化。结论本研究初步确定了载NGF纳米柔性脂质体的适宜制备条件,制备的脂质体具有较好稳定性。

Objective To study suitable preparation condition and stability of flexible nano-liposome （ FNL） loaded with nerve growth Factor （ NGF ） .Methods The FNL loaded with NGF was prepared by the method of filming and ultrasounding .The levels of NGF were detected by ELISA .The grain size of FNL was measured by laser grainsize analyzer . Results The optimal temperature of preparing FNL loaded with NGF was 35℃.The envelopment rate of prepared FNL peaked in pH 7.0.The suitable concentration of phosphatide was 40mg/ml.The best ratio of cholesterin：phosphatide was 1∶4.There was no obvious change in the grain size of FNL loaded with NGF after it was conserved in ambient temperature for 30 days and at 4℃in refrigerator for 12 months.Conclusion The study determines initially suitable preparation condition for FNL loaded with NGF,and prepared FNL has better stability .