三种不同IL-15基因转染对NCI-H446肿瘤细胞凋亡的影响

Effects of three kinds of IL-15 gene transfection on apoptosis of NCI-H446 cell

目的探讨不同形式IL-15基因转染对人小细胞肺癌细胞株NCI-H446细胞凋亡的影响。方法用构建的3种转染不同IL-15基因的NCI-H446细胞模型:原型(CIL-15)、成熟肽(CIL-15mp)及以IL-2信号肽替代IL-15信号肽的改型IL-15基因(CIL-2sp-IL-15mp),以野生株NCI-H446细胞(CW)为对照,流式细胞术检测细胞凋亡率及Fas蛋白表达。结果与CW相比,CIL-15、CIL-15mp和CIL-2sp-IL-15mp凋亡率及Fas蛋白表达依次增加(P<0.05或<0.01)。结论 3种IL-15基因转染均可致NCI-H446细胞凋亡率明显增加,强度依次为改型>成熟肽>原型;这种作用与增加NCI-H446细胞Fas蛋白的表达有关。

Objective To investigate the effect of three kinds of 1L-15 gene transfection on apoptosis of NCI-H446 cell. Methods The three kinds of NCI-H446 cell models were transfected respectively by IL-15 prototype gene, IL-15 mature peptide gene, IL-15 modified gene （CIL-2sp-IL-15 rap） in which the signal peptide of IL-15 was replaced with that of IL-2, and the three different kinds of IL-15 gene transfection models were regarded as experimental groups, besides,the wide NCI-H446 cells （Cw） were used as control group. The cell apoptosis rate and expression levels of Fas protein were detected by flow cytometry. Results As compared with those in control group, the cell apoptosis rate and expression levels of Fas protein were increased in CIL-15,CIL-15mp,CIL-2sp-IL-15mp in turn （ P 〈0. 05 or P 〈 0.01 ）. Conclusion The three kinds of IL-15 genes transfection can result in obvious increase of apoptosis rate of NCI-H446 cell,and the increase intensity is in turn IL-15 modified gene 〉 mature peptide gene 〉 prototype gene,which may be correlated with the increase of the expression levels of Fas protein.