HMGB-1在慢性肺栓塞患者血清中的表达及其与IL-1β、TNF-α、D-二聚体的相关性研究

Study on expression of HMGB-1 in chronic pulmonary embolism patients and its correlation with the expression of IL-1β,TNF-α and D-dimer

目的观测慢性肺栓塞(CPE)患者血清中HMGB-1的表达水平及其与IL-1β、TNF-α、D-二聚体表达水平的相关性。方法 CPE组选取CPE患者38例,对照组选取同期健康体检者42例。采用酶联免疫吸附(ELISA)方法检测2组血清中HMGB-1、IL-1β、TNF-α蛋白的表达水平,采用免疫比浊法测定血清中D-二聚体表达水平,采用荧光定量PCR方法检测外周血单核细胞(PBMC)中HMGB-1 mRNA的表达水平。结果 CPE组血清中HMGB-1、IL-1β、TNF-α、D-二聚体的表达均明显高于对照组(P均<0.05),且血清中HMGB-1蛋白水平与IL-1β、TNF-α、D-二聚体水平均呈正相关(P均<0.05﹚。CPE组外周血单核细胞HMGB-1 mRNA表达量明显高于健康对照组﹙P<0.05﹚。结论 HMGB-1的增高可作为CPE患者的一个潜在的辅助诊断的指标,其增高的机制可能是由于各种因素刺激的外周血单核细胞HMGB-1 mRNA转录增强所致。

Objective It is to observe the expression of HMGB - 1 in patients with chronic pulmonary embolism（ CPE） and its correlation with the expression of IL - 1β,TNF - α and D - dimer. Methods 38 patients of CPE were selected into CPE group and 42 healthy subjects in the same period in were selected into control group. The expressions of serum HMGB -1,IL - 1β,TNF - αwere detected by ELISA method. D - Dimer was detected by immunoturbidimetry. HMGB - 1 mRNA ex-pression was detected by Real-time PCR in PBMC. Results The expression levels of HMGB - 1,IL - 1β,TNF - α,D - Di-mer in CPE group were significantly higher than those in control group（P ﹤ 0. 05）and the level of HMGB - 1 protein in serum of CPE patients was positively correlated with IL - 1β,TNF - α,D - Dimer（P ﹤ 0. 05）. The expression of HMGB - 1 mRNA in CPE group was significantly higher than those in control group（ P ﹤ 0. 05）. Conclusion The increase in HMGB - 1 can serve as a potential diagnosis index for CPE and the mechanism may be of the HMGB - 1 mRNA transcription enhancement stimulated by other factors in CPE patients PBMC.