应用电喷雾质谱技术分析鉴定桑叶中黄酮类化合物

Application of electrospray mass spectrometry analysis and identification of fiavonoids in Morus alba L.

通过电喷雾多级串联质谱技术分析、鉴定了桑叶提取物中的槲皮素、山奈酚-7-葡萄糖苷、异槲皮苷、山奈酚-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷、槲皮素-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷和芦丁6种黄酮类化合物.根据一些特征的中性碎片丢失,提出了该类化合物的质谱裂解规律.通过得到的相对分子质量和中性碎片信息并与文献中的已知化合物比较,建立了这一类化合物快速鉴定的质谱新方法.

The structures of fiavonoids in the extract of Morus alba L.,quercetin,kaempferol-7-O-glucoside,isoquercitrin,kaempferol-3-O-（6″-O-acetyl）-β-D- glucopyranoside,quercetin-3-O-（6″-O-acetyl）-β-D-glucopyranoside and rutin were analyzed and identified via electrospray ionization multi-stage mass spectrometry（ESI-MSn）in the negative mde.Some interesting neutral losses were found in the experiment,the identification of the structures was based on the comparison of molecular ions,and the fragment ions obtained by ESI-MSn experiments with the data was reported in the literatures.