一种基于水解过程的多糖快速鉴别方法

A hydrolysis process based rapid identification approach for polysaccharides

应用生物传感器记录树舌、灵芝和虫草多糖水解过程的还原糖(还原性末端数)和葡萄糖含量的实时变化,研究水溶性多糖水解过程中还原糖(还原性末端数)和葡萄糖的水解过程变化规律。研究表明,同一种多糖水解图谱稳定,不同种多糖图谱特异性明显,通过水解过程图谱的比对分析,可以实现对树舌、灵芝和虫草多糖的快速鉴别。

We employed biosensors to record real-time variations of reducing sugar and glucose contents in hydrolysis process of Ganoderma applanatum Pat,Ganoderma lucidum and Cordyceps polysaccharide.We further investigated the variation law of the hydrolysis process of reducing sugar and glucose in hydrolysis process of water-soluble polysaccharide.Results show that hydrolysis process map is stable for the same kind of polysaccharide,but the specificity of hydrolysis process map is significant for different kinds of polysaccharide.Rapid identification of Ganoderma applanatum Pat,Ganoderma lucidum,and Cordyceps polysaccharide can be implemented by the comparison analysis of hydrolysis process map.