黄绿青霉菌不同培养基上产毒能力差异的实验研究

Experimental Observation on P.C.V. and Its Ability to Produce Toxin on Different Culture Medium

目的 观察黄绿青霉菌在不同培养基上产毒能力的变化情况。方法 用马铃薯葡萄糖培养基和查氏培养基分别培养黄绿青霉菌 ,然后提取培养基中的黄绿青霉毒素 ,经高效液相色谱检测毒素含量。结果 黄绿青霉菌在查氏培养基上生长旺盛 ,产毒能力大于在马铃薯葡萄糖培养基。结论 利用查氏培养基培养黄绿青霉菌可得到比较高的毒素产量。

Objective To observe the alteration of toxin producing ability of P.C.V. on different culture medium and Charles culture medium respectively. CIT in both culture medium was extracted and quantitated by HPL. Results P.C.V. was grown better on Charles culture medium than on potato glucose culture medium, and also produced more toxin. Conclusion Higher level of CIT could be obtained by culturing P.C.V.on Charles culture medium.