基于数字表达谱对茶树类黄酮合成、调控相关基因表达的研究

Analysis of Differential Gene Expression on Flavonoid Biosynthesis and Its Regulation in Tea Plant (Camellia sinensis L.)

利用数字基因表达谱技术研究了茶树类黄酮合成及调控相关基因在花瓣、花粉、休眠芽、萌发芽中的表达情况。结果发现花瓣、休眠芽、萌发芽中类黄酮合成基因大量表达,而花粉中类黄酮合成基因表达量极少,这与茶树中类黄酮物质的分布规律一致。同时本研究在类黄酮调控相关MYB、bHLH、MADS、GST、WD40、Homeodomain基因家族中找到12个基因可能与花瓣中类黄酮物质合成调控有关,9个基因可能与芽中类黄酮物质合成调控有关。这些研究结果将为深入解析茶树类黄酮合成、调控机理打下基础。

The expression of genes associated with flavonoid biosynthesis and its regulation in petal, pollen, dormant and sprouting buds of tea plants were analyzed by differential gene expression. The results showed that flavonoid biosynthetic genes were highly expressed in petal, dormant and sprouting buds, but rarely expressed in pollen, which is consistent with the distribution of flavonoids in tea plant. Furthermore, twelve genes in MYB, bHLH, MADS, GST, WD40 and Homeodomain gene families were identified to be correlated with flavonoid biosynthesis in petal. Nine genes were found to be involved in the regulation of flavonoid biosynthesis in tea buds. These results will be a basis for further understanding the mechanism of flavonoid biosynthetsis and regulation in Camellia sinensis.